Evaluation of biochemical markers in the golden mussel Limnoperna fortunei exposed to glyphosate acid in outdoor microcosms

• Published in: Ecotoxicology and environmental safety Vol. 95; pp. 123 - 129
• Main Authors: Iummato, María Mercedes, Di Fiori, Eugenia, Sabatini, Sebastián Eduardo, Cacciatore, Luis Claudio, Cochón, Adriana Cristina, Ríos de Molina, María del Carmen, Juárez, Ángela Beatriz
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01-09-2013; Elsevier
• Subjects: acetylcholinesterase; Acetylcholinesterase - metabolism; adverse effects; alkaline phosphatase; Animal, plant and microbial ecology; Animals; Applied ecology; Biological and medical sciences; Biomarkers; Biomarkers - metabolism; Bivalvia; catalase; Catalase - metabolism; Ecotoxicology, biological effects of pollution; Effects of pollution and side effects of pesticides on protozoa and invertebrates; Fundamental and applied biological sciences. Psychology; glutathione transferase; Glutathione Transferase - metabolism; Glycine - analogs & derivatives; Glycine - toxicity; Glyphosate; Golden mussel; Herbicides - toxicity; Limnoperna fortunei; lipid peroxidation; Lipid Peroxidation - drug effects; metabolism; Multibiomarker analysis; mussels; Mytilidae - drug effects; Mytilidae - metabolism; superoxide dismutase; Superoxide Dismutase - metabolism; Water Pollutants, Chemical - toxicity; Biomarkers; Limnoperna fortunei; Multibiomarker analysis; Glyphosate; Golden mussel; Oxidative stress; Edible mollusc; Mussel; Toxicity; Pesticides; Biological marker; Lipids; Organic phosphonate; Systemic; Antioxidant; Herbicide; Phosphorus Organic compounds; Enzymatic activity; Bivalvia; Aminoacid; Water pollution; Invertebrata; Mollusca; Peroxidation
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2013.05.024

In this study, the impact of technical grade glyphosate acid on Limnoperna fortunei was assessed employing outdoor microcosms treated with nominal glyphosate concentrations of 1, 3 and 6mgL−1. At the end of the experiment (26 days), catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST), acetylcholinesterase (AChE), carboxylesterases (CES) and alkaline phosphatase (ALP) activities, and lipid peroxidation levels were analyzed. GST and ALP activities and lipid peroxidation levels showed a significant increase with respect to controls in the mussels exposed to glyphosate (up to 90, 500 and 69 percent, respectively). CES and SOD activities showed a significant decrease in glyphosate exposed bivalves with respect to controls (up to 48 and 37 percent, respectively). CAT and AChE did not show differences between exposed and no exposed bivalves. The increase in lipid peroxidation levels and the decrease in SOD and CES activities observed in L. fortunei indicate that glyphosate had adverse effects on the metabolism of this bivalve. The results of the present study also indicate that a “multibiomarker approach” provides a more precise knowledge of the impact of glyphosate on L. fortunei.
•Impact of glyphosate acid on L. fortunei was assessed employing outdoor microcosms.•The exposition to glyphosate acid altered biochemical processes in L. fortunei.•TBARS increase and SOD and CES decrease indicate that glyphosate had adverse effects.•Multibiomarker approach provides knowledge of the impact of glyphosate on L. fortunei.