Mn-Containing Bioactive Glass-Ceramics: BMP-2-Mimetic Peptide Covalent Grafting Boosts Human-Osteoblast Proliferation and Mineral Deposition

• Published in: Materials Vol. 15; no. 13; p. 4647
• Main Authors: Cassari, Leonardo, Brun, Paola, Di Foggia, Michele, Taddei, Paola, Zamuner, Annj, Pasquato, Antonella, De Stefanis, Adriana, Valentini, Veronica, Saceleanu, Vicentiu Mircea, Rau, Julietta V., Dettin, Monica
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01-07-2022; MDPI
• Subjects: Aldehydes; Bioceramics; Bioglass; Biological activity; Biomedical materials; BMP-2; bone tissue engineering; Bones; Cell adhesion & migration; covalent functionalization; Deposition; Enzymes; Gene expression; Glass ceramics; glass-ceramic; Glycoproteins; Nitrates; Peptides; Scaffolds; Stem cells; Surgical implants; Tissue engineering; Wrist; Germany
• ISSN: 1996-1944; 1996-1944
• DOI: 10.3390/ma15134647

The addition of Mn in bioceramic formulation is gaining interest in the field of bone implants. Mn activates human osteoblast (h-osteoblast) integrins, enhancing cell proliferation with a dose-dependent effect, whereas Mn-enriched glasses induce inhibition of Gram-negative or Gram-positive bacteria and fungi. In an effort to further optimize Mn-containing scaffolds’ beneficial interaction with h-osteoblasts, a selective and specific covalent functionalization with a bioactive peptide was carried out. The anchoring of a peptide, mapped on the BMP-2 wrist epitope, to the scaffold was performed by a reaction between an aldehyde group of the peptide and the aminic groups of silanized Mn-containing bioceramic. SEM-EDX, FT-IR, and Raman studies confirmed the presence of the peptide grafted onto the scaffold. In in vitro assays, a significant improvement in h-osteoblast proliferation, gene expression, and calcium salt deposition after 7 days was detected in the functionalized Mn-containing bioceramic compared to the controls.