Production of structured lipids by acidolysis of an EPA-enriched fish oil and caprylic acid in a packed bed reactor: analysis of three different operation modes

• Published in: Biotechnology progress Vol. 20; no. 4; pp. 1044 - 1052
• Main Authors: Gonzalez Moreno, P.A, Robles Medina, A, Camacho Rubio, F, Camacho Paez, B, Molina Grima, E
• Format: Journal Article
• Language: English
• Published: USA American Chemical Society 01-07-2004; American Institute of Chemical Engineers
• Subjects: Acids - chemistry; Biological and medical sciences; Bioreactors; Biotechnology; Caprylates - chemistry; Eicosapentaenoic acid; Eicosapentaenoic Acid - chemistry; Enzymes, Immobilized - chemistry; Enzymes, Immobilized - metabolism; Fish oils; Fish Oils - chemistry; Fundamental and applied biological sciences. Psychology; Hydrolysis; Lipase - chemistry; Lipase - metabolism; Lipids; Lipids - chemistry; Liquid chromatography; Q1; Triglycerides; Triglycerides - chemistry; Lipids; Packed bed; Acidolysis; Fish oil; Production
• ISSN: 8756-7938; 1520-6033
• DOI: 10.1021/bp034314c

Structured triacylglycerols (ST) enriched in eicosapentaenoic acid (EPA) in position 2 of the triacylglycerol (TAG) backbone were synthesized by acidolysis of a commercially available EPA‐rich oil (EPAX4510, 40% EPA) and caprylic acid (CA), catalyzed by the 1,3‐specific immobilized lipase Lipozyme IM. The reaction was carried out in a packed bed reactor (PBR) operating in two ways: (1) by recirculating the reaction mixture from the exit of the bed to the substrate reservoir (discontinuous mode) and (2) in continuous mode, directing the product mixture leaving the PBR to a product reservoir. By operating in these two ways and using a simple kinetic model, representative values for the apparent kinetic constants (kX) for each fatty acid (native, Li or odd, M) were obtained. The kinetic model assumes that the rate of incorporation of a fatty acid into TAG per amount of enzyme, rX (mole/(h g lipase)) is proportional to the extent of the deviation from the equilibrium for each fatty acid (i.e., the difference of concentration between the fatty acid in the triacylglycerol and the concentration of the same fatty acid in the triacylglycerol once the equilibrium of the acidolysis reaction is reached). The model allows comparing the two operating modes through the processing intensity, defined as mLt/(V[TG]0) and mL/(q[TG]0), for the discontinuous and continuous operation modes, respectively. In discontinuous mode, ST with 59.5% CA and 9.6% EPA were obtained. In contrast, a ST with 51% CA and 19.6% EPA were obtained when using the continuous operation mode. To enhance the CA incorporation when operating in continuous mode, a two‐step acidolysis reaction was performed (third operation mode). This continuous two‐step process yields a ST with a 64% CA and a 15% EPA. Finally, after purifying the above ST in a preparative silica gel column, impregnated with boric acid, a ST with 66.9% CA and 19.6% EPA was obtained. The analysis by reverse phase and Ag+ liquid chromatography of the EPA‐enriched ST demonstrated that the CA was placed in positions 1 and 3 and the EPA was occupying position 2 of the final ST.