Characterization of viscerofugal neurons in human colon by retrograde tracing and multi-layer immunohistochemistry

• Published in: Frontiers in neuroscience Vol. 17; p. 1313057
• Main Authors: Chen, Bao Nan, Humenick, Adam G, Hibberd, Timothy James, Yew, Wai Ping, Wattchow, David A, Dinning, Phil G, Costa, Marcello, Spencer, Nick J, Brookes, Simon J H
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Research Foundation 16-01-2024; Frontiers Media S.A
• Subjects: Autonomic nervous system; Axons; Calbindin; Calcitonin gene-related peptide; Calretinin; Cell body; Coding; Colon; Cytology; Enkephalins; Enteric nervous system; Ganglia; Glucose; Glucose metabolism; Immunohistochemistry; intestinofugal; Labeling; Large intestine; Motility; Motor neurons; multiplexed immunohistochemistry; Myenteric plexus; Nerves; Neural networks; Neuropeptide Y; Neuroscience; Penicillin; Reflexes; Software; Substance P; Sympathetic nerves; viscerofugal; Australia; colon; enteric nervous system; viscerofugal; intestinofugal; multiplexed immunohistochemistry
• ISSN: 1662-4548; 1662-453X; 1662-453X
• DOI: 10.3389/fnins.2023.1313057

Viscerofugal neurons (VFNs) have cell bodies in the myenteric plexus and axons that project to sympathetic prevertebral ganglia. In animals they activate sympathetic motility reflexes and may modulate glucose metabolism and feeding. We used rapid retrograde tracing from colonic nerves to identify VFNs in human colon for the first time, using
preparations with multi-layer immunohistochemistry.
Colonic nerves were identified in isolated preparations of human colon and set up for axonal tracing with biotinamide. After fixation, labeled VFN cell bodies were subjected to multiplexed immunohistochemistry for 12 established nerve cell body markers.
Biotinamide tracing filled 903 viscerofugal nerve cell bodies (
= 23), most of which (85%) had axons projecting orally before entering colonic nerves. Morphologically, 97% of VFNs were uni-axonal. Of 215 VFNs studied in detail, 89% expressed ChAT, 13% NOS, 13% calbindin, 9% enkephalin, 7% substance P and 0 of 123 VFNs expressed CART. Few VFNs contained calretinin, VIP, 5HT, CGRP, or NPY. VFNs were often surrounded by dense baskets of axonal varicosities, probably reflecting patterns of connectivity; VAChT+ (cholinergic), SP+ and ENK+ varicosities were most abundant around them. Human VFNs were diverse; showing 27 combinations of immunohistochemical markers, 4 morphological types and a wide range of cell body sizes. However, 69% showed chemical coding, axonal projections, soma-dendritic morphology and connectivity similar to enteric excitatory motor neurons.
Viscerofugal neurons are present in human colon and show very diverse combinations of features. High proportions express ChAT, consistent with cholinergic synaptic outputs onto postganglionic sympathetic neurons in prevertebral ganglia.