Bioremediation of highly contaminated oilfield soil： Bioaugmentation for enhancing aromatic compounds removal

• Published in: Frontiers of environmental science & engineering Vol. 8; no. 2; pp. 293 - 304
• Main Authors: Qiao, Jun, Zhang, Chengdong, Luo, Shuiming, Chen, Wei
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01-04-2014; Higher Education Press; Springer Berlin Heidelberg; Springer Nature B.V
• Subjects: Aromatic compounds; Aromatic hydrocarbons; Bacteria; bioaugmentation; Biodegradation; Bioremediation; biostimulation; community structure; Compound fertilizers; Consortia; Earth and Environmental Science; Environment; Enzymatic activity; enzyme activity; fungi; Humic substances; Hydrocarbon-degrading bacteria; Hydrocarbons; Industrial wastes; microbial communities; Microorganisms; NPK fertilizers; Oil and gas fields; Oil fields; Organic wastes; Petroleum; petroleum hydrocarbon; Petroleum hydrocarbons; Physicochemical properties; Research Article; Soil contamination; Soil pollution; Soil properties; soil toxicity; Soils; synergism; Synergistic effect; Toxicity; Yeast; Yeasts; 微生物群落结构; 总石油烃; 氮磷钾复合肥; 污染土壤; 油田; 生物修复; 生物强化; 芳香族化合物; bioremediation; bioaugmentation; biostimulation; petroleum hydrocarbon
• ISSN: 2095-2201; 2095-221X
• DOI: 10.1007/s11783-013-0561-9

This study evaluated the effectiveness of different amendments—including a commercial NPK fertilizer, a humic substance (HS), an organic industrial waste (NovoGro), and a yeast-bacteria consortium—in the remediation of highly contaminated (up to 6% of total petroleum hydrocarbons) oilfield soils. The concentrations of hydrocarbon, soil toxicity, physicochemical properties of the soil, microbial population numbers, enzyme activities and microbial community structures were examined during the 90-d incubation. The results showed that the greatest degradation of total petroleum hydrocarbons (TPH) was observed with the biostimulation using mixture of NPK, HS and NovoGro, a treatment scheme that enhanced both dehydrogenase and lipase activities in soil. Introduction of exogenous hydrocarbon-degrading bacteria (in addition to biostimulation with NPK, HS and NovoGro) had negligible effect on the removal of TPH, which was likely due to the competition between exogenous and autochthonous microorganisms. Nonetheless, the addition of exogenous yeast-bacteria consortium significantly enhanced the removal of the aromatic fraction of the petroleum hydrocarbons, thus detoxifying the soil. The effect of bioaugmentation on the removal of more recalcitrant petroleum hydrocarbon fraction was likely due to the synergistic effect of bacteria and fungi.