Capillary liquid chromatographic determination of neutral phenolic compounds in apple juices

• Published in: Analytica chimica acta Vol. 426; no. 1; pp. 111 - 117
• Main Authors: Gomis, Domingo Blanco, Palomino, Natalia Fraga, Alonso, Juan José Mangas
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 2001; Elsevier
• Subjects: Analytical chemistry; Apple juices; Biological and medical sciences; Capillary liquid chromatography; Chemistry; Chromatographic methods and physical methods associated with chromatography; Exact sciences and technology; Food industries; Fundamental and applied biological sciences. Psychology; Non alcoholic beverage industries and mineral waters; Other chromatographic methods; Polyphenols; Polyphenols; Capillary liquid chromatography; Apple juices; Solid phase extraction; Chemical analysis; Liquid solid adsorption; HPLC chromatography; Fruit juice; Chemical enrichment; Polyphenol; Multicomponent analysis; Ultraviolet detector; Quality control; Benzenic compound; Beverage industry; Quantitative analysis
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/S0003-2670(00)01171-5

Capillary liquid chromatography (CLC) is evaluated as an alternative to conventional HPLC to analyse complex phenolics. Several neutral phenolic compounds were separated on a packed-reversed phase fused-silica capillary column, and determined with UV detection. A conventional liquid chromatographic equipment was adapted for such purposes. Application of the proposed method to the quantification of neutral phenols in apple juices is reported. Phenolics are extracted and fractionated into neutral and acidic compounds by means of a C
18 solid-phase cartridge. Typical recoveries ranging from 90 to 105% are obtained and reproducibility between extractions is <7% in all cases. Mass detection limits are at the sub-nanogram level.