Multiplex cell and lineage tracking with combinatorial labels

• Published in: Neuron (Cambridge, Mass.) Vol. 81; no. 3; pp. 505 - 520
• Main Authors: Loulier, Karine, Barry, Raphaëlle, Mahou, Pierre, Le Franc, Yann, Supatto, Willy, Matho, Katherine S, Ieng, Siohoi, Fouquet, Stéphane, Dupin, Elisabeth, Benosman, Ryad, Chédotal, Alain, Beaurepaire, Emmanuel, Morin, Xavier, Livet, Jean
• Format: Journal Article
• Language: English
• Published: United States Elsevier Limited 05-02-2014; Elsevier
• Subjects: Age Factors; Animals; Animals, Newborn; Bioengineering; Brain; Brain - cytology; Brain - embryology; Brain - growth & development; Cell Differentiation - physiology; Cell division; Cell Lineage - physiology; Cell Movement - physiology; Chick Embryo; Cloning; Color; Colorimetry; Deoxyribonucleic acid; DNA; Electroporation; Embryo, Mammalian; Genomes; Imaging; Labeling; Life Sciences; Luminescent Proteins - genetics; Luminescent Proteins - metabolism; Mice; Mice, Transgenic; Neurobiology; Neurogenesis - physiology; Neuroglia - physiology; Neurons - physiology; Neurons and Cognition; Optics; Physics; Spinal Cord - cytology; Spinal Cord - embryology; Stem Cells - cytology; Stem Cells - physiology; Time Factors; Transposases - physiology
• ISSN: 0896-6273; 1097-4199
• DOI: 10.1016/j.neuron.2013.12.016

We present a method to label and trace the lineage of multiple neural progenitors simultaneously in vertebrate animals via multiaddressable genome-integrative color (MAGIC) markers. We achieve permanent expression of combinatorial labels from new Brainbow transgenes introduced in embryonic neural progenitors with electroporation of transposon vectors. In the mouse forebrain and chicken spinal cord, this approach allows us to track neural progenitor's descent during pre- and postnatal neurogenesis or perinatal gliogenesis in long-term experiments. Color labels delineate cytoarchitecture, resolve spatially intermixed clones, and specify the lineage of astroglial subtypes and adult neural stem cells. Combining colors and subcellular locations provides an expanded marker palette to individualize clones. We show that this approach is also applicable to modulate specific signaling pathways in a mosaic manner while color-coding the status of individual cells regarding induced molecular perturbations. This method opens new avenues for clonal and functional analysis in varied experimental models and contexts.