Ligature‐induced peri‐implantitis and periodontitis in mice

• Published in: Journal of clinical periodontology Vol. 45; no. 1; pp. 89 - 99
• Main Authors: Hiyari, Sarah, Wong, Ryan L., Yaghsezian, Aline, Naghibi, Azadi, Tetradis, Sotirios, Camargo, Paulo M., Pirih, Flavia Q.
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01-01-2018
• Subjects: Acid phosphatase (tartrate-resistant); Alveolar bone; Animal models; Animals; Bone implants; Bone loss; Calcein; dental implant; Dental implants; Dental prosthetics; Dental restorative materials; Dentistry; Disease Models, Animal; Disease Progression; Gum disease; Ligation; ligature; Lymphocytes B; Male; Matrix metalloproteinase; Maxilla; Metalloproteinase; Mice; Mice, Inbred C57BL; Molars; murine model; Neutrophil collagenase; NF-κB protein; Osseointegration; Osteoclasts; Peri-Implantitis - etiology; Periodontitis; Periodontitis - etiology; peri‐implantitis; Teeth; Time Factors; Toluidine; murine model; ligature; peri-implantitis; dental implant; periodontitis
• ISSN: 0303-6979; 1600-051X
• DOI: 10.1111/jcpe.12817

Aim
Peri‐implantitis (PI), inflammation around dental implants, shares characteristics with periodontitis (PD). However, PI is more difficult to control and treat, and detailed pathophysiology is unclear. We aimed to compare PI and PD progression utilizing a murine model.
Materials and Methods
Four‐week‐old male C57BL/6J mice had their left maxillary molars extracted. Implants were placed in healed extraction sockets and osseointegrated. Ligatures were tied around the implants and second molars. Controls did not receive ligatures. Mice were sacrificed 1 week, 1 and 3 months (n ≥ 5/group/time point) post‐ligature placement. Bone loss analysis was performed. Histology was performed for: haematoxylin and eosin (H&E), tartrate‐resistant acid phosphatase (TRAP), matrix metalloproteinase‐8 (MMP‐8), nuclear factor kappa‐light‐chain enhancer of activated B cells (NF‐κB), toluidine blue and calcein.
Results
PI showed statistically greater bone loss compared to PD at 1 and 3 months. At 3 months, 20% of implants in PI exfoliated; no natural teeth exfoliated in PD. H&E revealed that alveolar bone surrounding implants in PI appeared less dense compared to PD. PI presented with increased osteoclasts, MMP‐8 and NF‐κB, compared to PD.
Conclusion
PI exhibited greater tissue and bone destruction compared to PD. Future studies will characterize the pathophysiological differences between the two conditions.