Moult-inhibiting fusion protein augments while polyclonal antisera attenuate moult stages and duration in Penaeus monodon

Highlights • Novel and innovative means of moult regulation in P. monodon with thioredoxin fused MIH I and antisera developed. • Report production of active thioredoxin-fused mature MIH I protein (mf-PmMIH I) of P. monodon for the first time in a bacterial system; and its use as antigen to raise pol...

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Bibliographic Details
Published in:	General and comparative endocrinology Vol. 233; pp. 32 - 42
Main Authors:	Sukumaran, Vrinda, Chekidhenkuzhiyil, Jasmin, Krishnankutty, Sivakumar C, Jose, Blessy, Philip, Rosamma, Singh, Isaac Sarojini Bright
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-07-2016
Subjects:	Animals Antibodies - immunology Antibodies - pharmacology Decapoda Endocrinology & Metabolism Escherichia coli Female Fusion recombinant protein Immune Sera - pharmacology Invertebrate Hormones - genetics Invertebrate Hormones - immunology Invertebrate Hormones - metabolism Invertebrate Hormones - pharmacology Mice Mice, Inbred BALB C MIH polyclonal antibody Molting - drug effects Molting - physiology Monodon Moult-inhibiting hormone (MIH) Penaeidae - drug effects Penaeidae - physiology Penaeus monodon Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Recombinant Fusion Proteins - pharmacology Thioredoxins - genetics Thioredoxins - immunology Thioredoxins - metabolism Thioredoxins - pharmacology Time Factors XO-SG complex Penaeus monodon MIH polyclonal antibody XO-SG complex Fusion recombinant protein Moult-inhibiting hormone (MIH)
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Summary:	Highlights • Novel and innovative means of moult regulation in P. monodon with thioredoxin fused MIH I and antisera developed. • Report production of active thioredoxin-fused mature MIH I protein (mf-PmMIH I) of P. monodon for the first time in a bacterial system; and its use as antigen to raise polyclonal antiserum (anti-mf-PmMIH I). • Mf-PmMIH I significantly augmented moult cycle duration by 16.67 ± 1.03 days at D2 stage and 14.67 ± 1.03 at D3 stage in comparison to the controls (11.67 ± 1.03 days). • Anti-mf-PmMIH I significantly attenuated moult duration to 8.33 ± 0.82 days with tendency to shift from C-stage to D0 – stage.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0016-6480 1095-6840
DOI:	10.1016/j.ygcen.2016.05.012