Identification of allergenic milk proteins markers in fined white wines by capillary liquid chromatography–electrospray ionization-tandem mass spectrometry

• Published in: Journal of Chromatography A Vol. 1217; no. 26; pp. 4300 - 4305
• Main Authors: Monaci, L., Losito, I., Palmisano, F., Visconti, A.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 25-06-2010; Amsterdam; New York: Elsevier; Elsevier
• Subjects: Allergens - analysis; Allergens - immunology; Biological and medical sciences; Caseins; Caseins - analysis; Caseins - immunology; Chromatography, Liquid - instrumentation; Chromatography, Liquid - methods; Fermented food industries; Food industries; Fundamental and applied biological sciences. Psychology; Liquid chromatography/tandem mass spectrometry; Milk allergens; Peptide-markers; Spectrometry, Mass, Electrospray Ionization - methods; Tandem Mass Spectrometry - methods; Vitaceae; Wine; Wine - analysis; Wines and vinegars; Wine; Liquid chromatography/tandem mass spectrometry; Milk allergens; Caseins; Peptide-markers; Capillary column; Tandem mass spectrometry; Peptides; White wine; Coupled method; HPLC chromatography; Biological marker; Electrospray; Milk protein; Casein; Peptide fragment; Phosphoproteins; Residue; Qualitative analysis; Whey protein; Allergen
• ISSN: 0021-9673; 1873-3778
• DOI: 10.1016/j.chroma.2010.04.035

A method based on capillary liquid chromatography combined with electrospray ionization-tandem mass spectrometry (CapLC–ESI-MS–MS) for the detection and identification of casein deriving peptides in fined white wine is described. This is the first step towards the development of a liquid chromatography mass spectrometric method for the detection/identification of markers of potentially allergenic milk proteins used as wine fining agents. The method demonstrated to be capable of detecting some peptides arising from α and β casein (with the relative aminoacidic sequences elucidated) in extracts of white wine fined with casein at 100 and 1000
μg/mL. This MS based approach appears to be a useful tool for screening purposes as well as a confirmatory tool for the unequivocal identification of caseins in ELISA positive samples.