Bacterial mediated green synthesis of silver nanoparticles and their antibacterial and antifungal activities against drug-resistant pathogens

• Published in: Royal Society open science Vol. 10; no. 10; p. 230796
• Main Authors: Huq, Md Amdadul, Khan, Azmat Ali, Alshehri, Jamilah M, Rahman, Md Shahedur, Balusamy, Sri Renukadevi, Akter, Shahina
• Format: Journal Article
• Language: English
• Published: England The Royal Society 25-10-2023
• Subjects: antimicrobial activity; Candida albicans; Chemistry; green synthesis; novel silver nanoparticles; Paenibacillus sp. MAHUQ-63; Salmonella Enteritidis; Candida albicans; novel silver nanoparticles; antimicrobial activity; green synthesis; Salmonella Enteritidis; Paenibacillus sp. MAHUQ-63
• ISSN: 2054-5703; 2054-5703
• DOI: 10.1098/rsos.230796

In the healthcare sector, the production of bioactive silver nanoparticles (AgNPs) with antimicrobial properties is of great importance. In this study, a novel bacterial strain,
sp. MAHUQ-63, was identified as a potential candidate for facile and rapid biosynthesis of AgNPs. The synthesized AgNPs were used to control the growth of human pathogens,
Enteritidis and
. The bacterial culture supernatant was used to synthesize the nanoparticles (NPs). Field emission transmission electron microscope examination showed spherical-shaped NPs with 15-55 nm in size. Fourier transform-infrared analysis identified various functional groups. The synthesized AgNPs demonstrated remarkable activity against
Enteritidis and
. The zones of inhibition for 100 µl (0.5 mg ml
) of AgNPs against
Enteritidis and
were 18.0 ± 1.0 and 19.5 ± 1.3 mm, respectively. The minimum inhibitory concentrations were 25.0 and 12.5 µg ml
against
Enteritidis and
, respectively. Additionally, the minimum bactericidal concentrations were 25.0 µg ml
against both pathogenic microbes. The field emission scanning electron microscopy analysis showed that the treatment of AgNPs caused morphological and structural damage to both
Enteritidis and
. Therefore, these AgNPs can be used as a new and effective antimicrobial agent.