A highly specific and ultrasensitive p-aminophenylether-based fluorescent probe for imaging native HOCl in live cells and zebrafish

• Published in: Analytica chimica acta Vol. 1052; pp. 131 - 136
• Main Authors: Jia, Pan, Zhuang, Zihan, Liu, Caiyun, Wang, Zuokai, Duan, Qingxia, Li, Zilu, Zhu, Hanchuang, Du, Bin, Zhu, Baocun, Sheng, Wenlong, Kang, Baotao
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 04-04-2019; Elsevier BV
• Subjects: Animals; Bioimaging; Cell Survival; Danio rerio; Fluorescent Dyes - metabolism; Fluorescent indicators; Fluorescent probe; Hydroxynaphthalimide; Hypochlorous acid; Hypochlorous acid (HOCl); Hypochlorous Acid - metabolism; Immune system; Limit of Detection; Mice; Optical Imaging; P-aminophenylether; Pathogenesis; Phenyl Ethers - metabolism; RAW 264.7 Cells; Zebrafish; Hydroxynaphthalimide; Bioimaging; Hypochlorous acid (HOCl); P-aminophenylether; Fluorescent probe
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2018.11.031

It is very important to detect native hypochlorous acid (HOCl) in the complex biosystems owing to the important roles of HOCl in the immune defense and the pathogenesis of numerous diseases. In this paper, a new p-aminophenylether-based fluorescent probe PAPE-HA was developed for specific detection of HOCl. Probe PAPE-HA could implement the quantitative detection of HOCl ranging from 0 to 1 μM and the detection limit was obtained as low as 1.37 nM. Additionally, probe PAPE-HA could reach a rapid response for HOCl (<2 min). Importantly, probe PAPE-HA with preeminent specificity and ultrasensitivity was proven to possess powerful capability of tracking native HOCl in live cells and zebrafish, and we thus anticipate that probe PAPE-HA could be used as a novel promising tool for revealing diverse cellular functions of HOCl.
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•A new p-aminophenylether-based fluorescent probe for tracking HOCl was developed.•The probe could sensitively determine HOCl (DL = 1.37 nM).•The probe exhibited high selectivity towards HOCl over •OH and ONOO−•The probe was applied to the visualization of native HOCl in live cells and zebrafish.