Ten antenna proteins are associated with the core in the supramolecular organization of the photosystem I supercomplex in Chlamydomonas reinhardtii

• Published in: The Journal of biological chemistry Vol. 294; no. 12; pp. 4304 - 4314
• Main Authors: Kubota-Kawai, Hisako, Burton-Smith, Raymond N., Tokutsu, Ryutaro, Song, Chihong, Akimoto, Seiji, Yokono, Makio, Ueno, Yoshifumi, Kim, Eunchul, Watanabe, Akimasa, Murata, Kazuyoshi, Minagawa, Jun
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 22-03-2019; American Society for Biochemistry and Molecular Biology
• Subjects: Chlamydomonas; Chlamydomonas reinhardtii; Chlamydomonas reinhardtii - metabolism; Chlorophyll - metabolism; cryo-electron microscopy; cryo-electron microscopy (cryo-EM); Crystallography, X-Ray; green algae; LHCI; light-harvesting complex (antenna complex); Light-Harvesting Protein Complexes - chemistry; Light-Harvesting Protein Complexes - metabolism; outer antenna; photosynthesis; photosynthetic efficiency; photosystem I; Photosystem I Protein Complex - chemistry; Photosystem I Protein Complex - metabolism; Plant Biology; single-particle analysis; Spectrometry, Fluorescence; light-harvesting complex (antenna complex); LHCI; photosynthetic efficiency; green algae; outer antenna; photosynthesis; Chlamydomonas reinhardtii; cryo-electron microscopy (cryo-EM); photosystem I; Chlamydomonas; cryo-electron microscopy; single-particle analysis
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.RA118.006536

Photosystem I (PSI) is a large pigment–protein complex mediating light-driven charge separation and generating a highly negative redox potential, which is eventually utilized to produce organic matter. In plants and algae, PSI possesses outer antennae, termed light-harvesting complex I (LHCI), which increase the energy flux to the reaction center. The number of outer antennae for PSI in the green alga Chlamydomonas reinhardtii is known to be larger than that of land plants. However, their exact number and location remain to be elucidated. Here, applying a newly established sample purification procedure, we isolated a highly pure PSI–LHCI supercomplex containing all nine LHCA gene products under state 1 conditions. Single-particle cryo-EM revealed the 3D structure of this supercomplex at 6.9 Å resolution, in which the densities near the PsaF and PsaJ subunits were assigned to two layers of LHCI belts containing eight LHCIs, whereas the densities between the PsaG and PsaH subunits on the opposite side of the LHCI belt were assigned to two extra LHCIs. Using single-particle cryo-EM, we also determined the 2D projection map of the lhca2 mutant, which confirmed the assignment of LHCA2 and LHCA9 to the densities between PsaG and PsaH. Spectroscopic measurements of the PSI–LHCI supercomplex suggested that the bound LHCA2 and LHCA9 proteins have the ability to increase the light-harvesting energy for PSI. We conclude that the PSI in C. reinhardtii has a larger and more distinct outer-antenna organization and higher light-harvesting capability than that in land plants.