Effects of sodium chloride on the gene expression profile of periodontal ligament fibroblasts during tensile strain

• Published in: Journal of orofacial orthopedics Vol. 81; no. 5; pp. 360 - 370
• Main Authors: Schröder, Agnes, Gubernator, Joshua, Nazet, Ute, Spanier, Gerrit, Jantsch, Jonathan, Proff, Peter, Kirschneck, Christian
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer Medizin 01-09-2020
• Subjects: Cells, Cultured; Dentistry; Fibroblasts; Medicine; Oral and Maxillofacial Surgery; Original; Original Article; Periodontal Ligament; Sodium Chloride; Stress, Mechanical; Tooth Movement Techniques; Transcriptome; Parodontalligament-Fibroblasten; Orthodontic tooth movement; Parodontalligament (PDL); Tensile strain; Periodontal ligament (PDL); Genexpression; Periodontal ligament fibroblast; Dehnung; Kieferorthopädische Zahnbewegung; Expression kinetics
• ISSN: 1434-5293; 1615-6714
• DOI: 10.1007/s00056-020-00232-8

Purpose
During orthodontic tooth movement, pressure and tension zones develop in the periodontal ligament, and periodontal ligament fibroblasts (PDLF) become exposed to mechanical strain. Enhanced salt (NaCl) concentrations are known to modulate responses of PDLF and immune cells to different stimuli like mechanical strain. Here, we investigated the impact of tensile strain on the gene expression profile of PDLF under normal (NS) and high salt (HS) conditions.
Methods
After preincubation under NS or HS (+40 mM NaCl in medium) conditions for 24 h, PDLF were stretched 16% for 48 h using custom-made spherical cap silicone stamps using an established and published setup. After determination of cell number and cytotoxicity, we analyzed expression of genes involved in extracellular matrix reorganization, angiogenesis, bone remodeling, and inflammation by quantitative real-time polymerase chain reaction (RT-qPCR).
Results
Tensile strain did not affect the expression of genes involved in angiogenesis or extracellular matrix reorganization by PDLF, which however modulate inflammatory responses and bone remodeling in reaction to 16% static tensile strain. Salt (NaCl) treatment triggered enhanced extracellular matrix formation, expression of cyclooxygenase 2 and bone metabolism in PDLF during tensile strain.
Conclusions
Salt (NaCl) consumption may influence orthodontic tooth movement and periodontal bone loss via modulation of extracellular matrix and bone metabolism. Excessive salt intake during orthodontic therapy may cause adverse effects regarding periodontal inflammation and bone resorption.