Fabrication of Anti-human Cardiac Troponin I Immunogold Nanorods for Sensing Acute Myocardial Damage

• Published in: Nanoscale research letters Vol. 4; no. 12; pp. 1428 - 1433
• Main Authors: Guo, ZR, Gu, CR, Fan, X, Bian, ZP, Wu, HF, Yang, D, Gu, N, Zhang, JN
• Format: Journal Article
• Language: English
• Published: New York Springer New York 01-12-2009; Springer Nature B.V; Springer
• Subjects: Chemistry and Materials Science; Materials Science; Molecular Medicine; Nano Express; Nanochemistry; Nanoscale Science and Technology; Nanotechnology; Nanotechnology and Microengineering; Surface modification; Gold nanorod; Cardiac troponin I; Biosensor
• ISSN: 1556-276X; 1931-7573; 1556-276X
• DOI: 10.1007/s11671-009-9415-6

A facile, rapid, solution-phase method of detecting human cardiac troponin I for sensing myocardial damage has been described using gold nanorods-based biosensors. The sensing is demonstrated by the distinct change of the longitudinal surface plasmon resonance wavelength of the gold nanorods to specific antibody–antigen binding events. For a higher sensitivity, the aspect ratio of gold nanorods is increased up to ca 5.5 by simply adding small amount of HCl in seed-mediated growth solution. Experimental results show that the detecting limit of the present method is 10 ng/mL. Contrast tests reveal that these gold nanorods-based plasmonic biosensors hold much higher sensitivity than that of conventionally spherical gold nanoparticles.