Investigating the effects of a sub-lethal metal mixture of Cu, Zn and Cd on bioaccumulation and ionoregulation in common carp, Cyprinus carpio

• Published in: Aquatic toxicology Vol. 218; p. 105363
• Main Authors: Castaldo, G., Pillet, M., Slootmaekers, B., Bervoets, L., Town, R.M., Blust, R., De Boeck, G.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-01-2020; Elsevier
• Subjects: Animals; Bioaccumulation - genetics; Biodiversity and Ecology; Biomarkers - metabolism; Cadmium - metabolism; Cadmium - toxicity; Carps - genetics; Carps - metabolism; Copper - metabolism; Copper - toxicity; Cyprinus carpio; Ecosystem; Ecotoxicology; Electrolytes - metabolism; Environmental Sciences; Gene Expression - drug effects; Homeostasis - drug effects; Ion-homeostasis; Ionoregulation; Lethal Dose 50; Life Sciences; Metal pollution; Metallothionein - genetics; Mixture stress; Proton-Translocating ATPases - genetics; Toxicology; Water Pollutants, Chemical - metabolism; Water Pollutants, Chemical - toxicity; Zinc - metabolism; Zinc - toxicity; Cyprinus carpio; Ionoregulation; Mixture stress; Ion-homeostasis; Metal pollution
• ISSN: 0166-445X; 1879-1514
• DOI: 10.1016/j.aquatox.2019.105363

•Common carp exposed to environmentally relevant concentrations of copper (Cu), zinc (Zn) and cadmium (Cd).•Significant Cu and Cd accumulation in the gills, whereas Zn levels remained stable.•General Na decrease in all tissues counteracted by induction of H+-ATPase gene expression.•Strong induction of metallothionein gene expression.•Antagonistic mutual inhibition of Cd and Zn uptake.
The aquatic environment is continuously under threat because it is the final receptor and sink of waste streams. The development of industry, mining activities and agriculture gave rise to an increase in metal pollution in the aquatic system. Thus a wide occurrence of metal mixtures exists in the aquatic environment. The assessment of mixture stress remains a challenge considering that we can not predict the toxicity of a mixture on the basis of single compounds. Therefore the analysis of the effects of environmentally relevant waterborne mixtures is needed to improve our understanding of the impact of metal pollution in aquatic ecosystems. Our aim was to assess whether 10 % of the concentration of the 96 h LC50 (the concentration that is lethal to 50 % of the population in 96 h) of individual metal exposures can be considered as a “safe” concentration when applied in a trinomial mixture. Therefore, common carp were exposed to a sublethal mixture of Cu 0.07 ± 0.001 μM (4.3 ± 0.6 μg/L), Zn 2.71 ± 0.81 μM (176.9 ± 52.8 μg/L) and Cd 0.03 ± 0.0004 μM (3.0 ± 0.4 μg/L) at 20 °C for a period of one week. Parameters assessed included survival rate, bioaccumulation and physiological biomarkers related to ionoregulation and defensive mechanisms such as MT induction. Our results showed a sharp increase in Cu and Cd concentration in gills within the first day of exposure while Zn levels remained stable. The accumulation of these metals led to a Na drop in gills, liver and muscle as well as a decreased K content in the liver. Biomarkers related to Na uptake were also affected: on the first day gene expression for H+-ATPase was transiently increased while a concomitant decreased gene expression of the Na+/H+ exchanger occurred. A fivefold induction of metallothionein gene expression was reported during the entire duration of the experiment. Despite the adverse effects on ionoregulation all fish survived, indicating that common carp are able to cope with these low metal concentrations, at least during a one week exposure.