Effect of interleukin-12 and interleukin-10 on the virus replication and apoptosis in T-cells infected with feline immunodeficiency virus

• Published in: Journal of Veterinary Medical Science Vol. 60; no. 11; pp. 1181 - 1185
• Main Authors: Mortola, E. (Tokyo Univ. (Japan)), Endo, Y, Mizuno, T, Ohno, K, Watari, T, Tsujimoto, H, Hasegawa, A
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 1998; Japan Science and Technology Agency
• Subjects: Adjuvants, Immunologic - pharmacology; AIDS/HIV; Animals; APOPTOSE; APOPTOSIS; Apoptosis - drug effects; CATS; Cell Line; CHAT; Feline immunodeficiency virus; FIV; GATO; Humans; IMMUNODEFICIENCE; IMMUNODEFICIENCY; Immunodeficiency Virus, Feline - physiology; INMUNODEFICIENCIA; interleukin-10; Interleukin-10 - pharmacology; interleukin-12; Interleukin-12 - pharmacology; INTERLEUKINE; INTERLEUKINS; INTERLEUQUINAS; Lentivirus Infections - veterinary; Lentivirus Infections - virology; Recombinant Proteins - pharmacology; REPLICACION; REPLICATION; T-Lymphocytes - virology; Virus Replication - drug effects
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.60.1181

Interleukin 12 (IL-12) is an important cytokine for Th 1 response which stimulates the T-cell population to produce cytokines for cellular immunity. Interleukin-10 (IL-10) is a pleiotropic cytokine capable of suppressing cytokine production from macrophages and T-cells and participates in Th 2 immune response. The present study was carried out to examine the effect of these cytokines on virus replication and Apoptosis in T-cells infected with feline immunodeficiency virus (FIV). Infection of a feline T-lymphoid cell line (Fel-039) resulted in an increase of the reverse transcriptase (RT) activity in the culture supernatant accompanied by cell death from apoptosis. Addition of human recombinant IL-12 significantly inhibited the virus replication and apoptosis in Fel-039 cells in a dose dependent manner. Furthermore, the antiviral activity of IL-12 was associated with the expression of IFN-gamma in the FIV-infected Fel-039 cells. In contrast, human recombinant IL-10 did not show any inhibitory effect on the virus replication and apoptosis in the Fel-039 cells infected with FIV. These results suggest that the inhibitory effect of IL-12 on both virus replication and apoptosis has potential implications for the design of immunotherapy strategies using IL-12 in FIV infection