Elucidating Flavonoid and Antioxidant Activity in Edible and Medicinal Herbs Woodwardia japonica (L.f.) Sm. Based on HPLC-ESI-TOF-MS and Artificial Neural Network Model: Response to Climatic Factors

• Published in: Molecules (Basel, Switzerland) Vol. 28; no. 4; p. 1985
• Main Authors: Wang, Xin, Cao, Jianguo, Tian, Lin, Liu, Baodong, Fan, Yawen, Wang, Quanxi
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 20-02-2023; MDPI
• Subjects: Algorithms; antioxidant activities; Antioxidants; Antioxidants - pharmacology; artificial neural network model; Chromatography, High Pressure Liquid; Climate; Climate effects; climate factors; Cluster analysis; Colorimetry; edible and medicinal fern; Ferns; Flavones; Flavonoids; Flavonoids - pharmacology; Flavonols; Herbal medicine; High-performance liquid chromatography; Humidity; Isoflavones; Liquid chromatography; Low temperature; Mathematical models; Medicinal herbs; Metabolites; Neural networks; Plant Extracts - pharmacology; Plants, Medicinal; Precipitation; Secondary metabolites; Spectrophotometry; Statistical methods; total flavonoid content; Woodwardia; Woodwardia japonica; Jiangxi China; China; Woodwardia japonica; artificial neural network model; antioxidant activities; climate factors; total flavonoid content; edible and medicinal fern
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules28041985

is a kind of great potential edible and medicinal fern. In a previous study, it was found that flavonoid and antioxidant activity of
from different sites were different. However, the cause of the differences has still been unclear, which has restricted the utilization of
. In this paper, flavonoid and antioxidant activity of
from nine different regions were determined with the method of a colorimetric assay with UV-VIS spectrophotometry and HPLC-ESI-TOF-MS, and the effects of climate factors on flavonoids and antioxidant activities were evaluated by mathematical modeling and statistical methods. The results showed: (1) total flavonoid content (TFC) of
from Wuyi Mountain (Jiangxi) was the highest, which might be related to the low temperature; (2) the differences of antioxidant activities of
might be related to precipitation; (3) five flavonols, two flavones and one isoflavone were tentatively identified in
; (4) flavonol and isoflavone might be affected by sunshine duration, and flavones were probably related to temperature. In conclusion, the effects of climate factors on flavonoids and antioxidants are significant, which would provide an important basis for further exploring the mechanism of climate affecting secondary metabolites.