Morphological and molecular characterization of Isospora neochmiae n. sp. in a captive-bred red-browed finch (Neochmia temporalis) (Latham, 1802)

• Published in: Experimental parasitology Vol. 166; pp. 181 - 188
• Main Authors: Yang, Rongchang, Brice, Belinda, Ryan, Una
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-07-2016
• Subjects: 18S rRNA; 28S rRNA; Animals; Apicomplexa; Apodemus flavicollis; Australia - epidemiology; Base Sequence; Bird Diseases - epidemiology; Bird Diseases - parasitology; COI; Columba livia; DNA, Protozoan - chemistry; DNA, Protozoan - isolation & purification; Eimeriidae; Electron Transport Complex IV - genetics; Evolution, Molecular; Finches - parasitology; hsp70; HSP70 Heat-Shock Proteins - genetics; Isospora; Isospora - classification; Isospora - genetics; Isospora - ultrastructure; Isosporiasis - epidemiology; Isosporiasis - parasitology; Isosporiasis - veterinary; Jejunum - parasitology; Mitochondria - enzymology; Morphology; Oocysts - ultrastructure; Passer; Passer domesticus; Phylogeny; Psarocolius; Red-browed finch; RNA, Ribosomal, 18S - genetics; RNA, Ribosomal, 28S - genetics; Sequence Alignment - veterinary; hsp70; Red-browed finch; Morphology; 18S rRNA; 28S rRNA; Isospora; Phylogeny; COI
• ISSN: 0014-4894; 1090-2449
• DOI: 10.1016/j.exppara.2016.04.011

A new Isospora (Apicomplexa: Eimeriidae) species is described from a single red-browed finch (Neochmia temporalis) (subspecies N. temporalis temporalis), that was part of a captive population in Western Australia. Sporulated oocysts of this isolate are spherical, 18.3 (18.2–18.9) × 18.2 (18.2–18.6) μm, with a shape index (length/width) of 1.0; and a smooth and bilayered oocyst wall, 1.2 μm thick (outer layer 0.9 μm, inner 0.3 μm). A polar granule is present, but the oocyst residuum and a micropyle are absent. The sporocysts are ovoid-shaped, 13.3 (9.5–16.4) × 8.6 (6.8–10.0) μm, with a shape index of 1.5. An indistinct Stieda body is present, but the substieda body is absent. A sporocyst residuum is present and composed of numerous granules of different size scattered among the sporozoites. Morphologically, the oocysts from this isolate are different from those of all known valid Isospora spp. Molecular analysis was conducted at 4 loci; the 18S and 28S ribosomal RNA (rRNA), the mitochondrial cytochrome oxidase (COI) gene and the heat shock protein 70 (hsp70) gene. At the 18S locus, this new isolate exhibited 99.9%, 99.8%, 99.7%, and 99.5% similarity to I. sp. MAH-2013a from a superb starling (Lamprotornis superbus), I. MS-2003 from a Southern cape sparrow (Passer melanurus), I. sp. Tokyo from a domestic pigeon (Columba livia domestica) and I. MS-2003 from a Surinam crested oropendula (Psarocolius decumanus). At the 28S locus, this new isolate exhibited 99.7% similarity to both an Isospora sp (MS-2003) from a Northern house sparrow (Passer domesticus) and an Isospora sp. (MS-2003) from a Southern cape sparrow. At the COI locus, this new isolate exhibited 98.9% similarity to an Isospora sp. ex Apodemus flavicollis. At the hsp70 locus, this new isolate exhibited 99% similarity to isolate MS-2003 (AY283879) from a wattled starling (Creatophora cinerea). Based on morphological and molecular data, this isolate is a new species of Isospora, which is named Isospora neochmiae n. sp. after its host, the red-browed finch (Neochmia temporalis).
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•A new Isospora species (Isospora neochmiae n. sp.) in a red-browed finch.•Morphology study distinct to other validated Isospora species.•Genetic study: Most close to I. sp. MAH-2013a (KF648870) at 18S locus.