Evaluation of MCT1, MCT4 and CD147 Genes in Peripheral Blood Cells of Breast Cancer Patients and Their Potential Use as Diagnostic and Prognostic Markers

• Published in: International journal of molecular sciences Vol. 18; no. 4; p. 170
• Main Authors: Luz, Maria Cláudia de B, Perez, Matheus M, Azzalis, Ligia A, Sousa, Luiz Vinícius de A, Adami, Fernando, Fonseca, Fernando L A, Alves, Beatriz da C A
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01-04-2017; MDPI
• Subjects: Acidosis; Adult; Aged; Biological properties; Biomarkers; Biomarkers, Tumor - blood; Blood cells; Breast cancer; Breast Neoplasms - blood; Case-Control Studies; CD146 Antigen - blood; CD147 antigen; Diagnosis; Diagnostic systems; Female; Genes; Humans; Hypoxia; Markers; Membrane proteins; Metabolic acidosis; Metastases; Metastasis; Middle Aged; Monocarboxylic Acid Transporters - blood; Muscle Proteins - blood; Oxidative stress; Patients; Peripheral blood; Symporters - blood; Tumor cells; hypoxia; breast cancer; metastasis; prognosis; biomarkers; monocarboxylate transporter (MCT)
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms18040170

Patients with breast cancer-the deadliest cancer among women-are at constant risk of developing metastasis. Oxidative stress and hypoxia are common feature of tumor cells that can proliferate even in a resultant metabolic acidosis. Despite the low extracellular pH, intracellular pH of tumor cells remains relatively normal, or even more alkaline due to the action of a membrane protein family known as monocarboxylate transporters (MCTs). The objective of this study was to verify the diagnostic and prognostic value of
,
and
in tumor and peripheral blood samples of patients with breast cancer undergoing chemotherapic treatment.
Differential expression of
,
and
obtained by qPCR was determined by 2
method between biological samples (tumor and serial samples of peripheral) of patients (
= 125) and healthy women (
= 25).
tumor samples with higher histological grades have shown higher expression of these markers; this higher expression was also observed in blood samples obtained at diagnosis of patients when compared to healthy women and in patients with positive progression of the disease (metastasis development).
markers studied here could be a promising strategy in routine laboratory evaluations as breast cancer diagnosis and prognosis.