The Coxsackievirus and Adenovirus Receptor Has a Short Half-Life in Epithelial Cells

• Published in: Pathogens (Basel) Vol. 11; no. 2; p. 173
• Main Authors: Kotha Lakshmi Narayan, Poornima, Readler, James M, Alghamri, Mahmoud S, Brockman, Trisha L, Yan, Ran, Sharma, Priyanka, Snitsarev, Vladislav, Excoffon, Katherine J D A, Kolawole, Abimbola O
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 27-01-2022; MDPI
• Subjects: Adenoviruses; Cell adhesion; Cell signaling; Communication; coxsackievirus and adenovirus receptor; Coxsackieviruses; Epithelial cells; Epithelium; Half-life; human adenovirus; Infections; Localization; polarized epithelia; Proteins; Receptors; Signal transduction; Viral infections; polarized epithelia; human adenovirus; coxsackievirus and adenovirus receptor; half-life
• ISSN: 2076-0817; 2076-0817
• DOI: 10.3390/pathogens11020173

The coxsackievirus and adenovirus receptor (CAR) is an essential cellular protein that is involved in cell adhesion, cell signaling, and viral infection. The 8-exon encoded isoform (CAR
) resides at the apical surface of polarized epithelia, where it is accessible as a receptor for adenovirus entering the airway lumen. Given its pivotal role in viral infection, it is a target for antiviral strategies. To understand the regulation of CAR
and determine the feasibility of receptor downregulation, the half-life of total and apical localized CAR
was determined and correlated with adenovirus transduction. Total and apical CAR
has a relatively short half-life of approximately 2 h. The half-life of apical CAR
correlates well with adenovirus transduction. These results suggest that antiviral strategies that aim to degrade the primary receptor for apical adenovirus infection will be effective within a relatively short time frame after application.