Association of the long pentraxin PTX3 gene polymorphism (rs3816527) with migraine in an Iranian population

• Published in: Journal of the neurological sciences Vol. 349; no. 1; pp. 185 - 189
• Main Authors: Zandifar, Alireza, Iraji, Niloufar, Taheriun, Maryam, Tajaddini, Mohamadhasan, Javanmard, Shaghayegh Haghjooy
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15-02-2015
• Subjects: Adult; C-Reactive Protein - genetics; Case-Control Studies; Female; Gene Frequency; Gene polymorphism; Genetic Predisposition to Disease; Genotype; Headache severity; HIT-6; Humans; Male; Migraine; Migraine Disorders - genetics; Migraine Disorders - physiopathology; Neurology; Pentraxin; Polymorphism, Single Nucleotide; PTX3; Serum Amyloid P-Component - genetics; Severity of Illness Index; Sex Factors; Surveys and Questionnaires; CRP; NO; HRM; C-reactive protein; HIT-6; CNS; headache impact test; TNF; Gene polymorphism; LPS; high-resolution melt; SNP; analysis of variance; Hardy–Weinberg equilibrium; IHS; ANOVA; cystic fibrosis; HWE; international headache society; TLR; single-nucleotide polymorphism; Toll-like receptor; polymerase chain reaction; Pentraxin; Headache severity; nitric oxide; CF; CI; Migraine; tuberculosis; TB; interleukins; central nervous system; ILs; tumor necrosis factor; PTX3; pentaxins 3; confidence interval; lipopolysaccharide; PCR
• ISSN: 0022-510X; 1878-5883
• DOI: 10.1016/j.jns.2015.01.015

Abstract Background Migraine is a common neurovascular disorder with multifactorial and polygenic inheritance. It has been shown that migraine may be a form of sterile neurogenic inflammation. Pentaxins 3 (PTX3) has been detected in brain during inflammatory responses. The aim of our study was to investigate the association of rs3816527 polymorphism of the PTX3 gene and migraine in an Iranian population. Method We included 103 newly diagnosed migraine patients and 148 healthy subjects as control group. Genomic DNA samples extracted from the peripheral blood and genotypes of PTX3 rs3816527 gene polymorphism were determined. The patients filled out HIT-6 questionnaire as a scale to evaluate the severity of headache. Results The genotype frequency of PTX3 was significantly different between the migraine patients and the control subjects. CC variant homozygote genotype was statistically more frequent in the patients than in the controls (P < 0.05; OR = 1.74, 95% CI = 1.04–2.94). Also the C allele was not significantly more frequent in the patients (P = 0.096; OR = 1.27, 95% CI = 0.88–1.85). A separate analysis in male and female subjects showed no significant differences between the different genotypes and phenotypes of PTX3 rs3816527 gene and susceptibility to migraine in female subjects. Total HIT-6 score was significantly different between three PTX3 genotypes (P = 0.008). Conclusion In conclusion our results showed the association between the PTX3 rs3816527 gene polymorphism with susceptibility to migraine only in the male patients. Also total HIT-6 scores as a scale for assessment of the severity were related to the PTX3 rs3816527 gene polymorphism. But this relation was not established by headache frequency.