Synaptic scaffolding molecule interacts with Axin

• Published in: Journal of neurochemistry Vol. 90; no. 2; pp. 332 - 339
• Main Authors: Hirabayashi, Susumu, Nishimura, Wataru, Iida, Junko, Kansaku, Ai, Kishida, Shosei, Kikuchi, Akira, Tanaka, Noriaki, Hata, Yutaka
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01-07-2004; Blackwell
• Subjects: Adaptor Proteins, Signal Transducing; Animals; Axin; Axin Protein; beta Catenin; Binding, Competitive - physiology; Biological and medical sciences; Brain Chemistry; Carrier Proteins - chemistry; Carrier Proteins - genetics; Carrier Proteins - metabolism; Cell physiology; Cells, Cultured; Central nervous system; Central neurotransmission. Neuromudulation. Pathways and receptors; Cercopithecus aethiops; Cytoskeletal Proteins - chemistry; Cytoskeletal Proteins - metabolism; Fundamental and applied biological sciences. Psychology; glycogen synthase kinase; Glycogen Synthase Kinase 3 - chemistry; Glycogen Synthase Kinase 3 - metabolism; Glycogen Synthase Kinase 3 beta; Guanylate Kinases; Hippocampus - cytology; Humans; Macromolecular Substances; Molecular and cellular biology; Mutagenesis, Site-Directed; Neurons - metabolism; Neurotransmission; Phosphorylation - drug effects; Precipitin Tests; Protein Binding - physiology; Protein Structure, Tertiary - physiology; Rats; Repressor Proteins - chemistry; Repressor Proteins - genetics; Repressor Proteins - metabolism; Subcellular Fractions - chemistry; Synapses - metabolism; synaptic scaffolding molecule; Trans-Activators - chemistry; Trans-Activators - metabolism; Transfection; Two-Hybrid System Techniques; Vertebrates: nervous system and sense organs; β‐catenin; Phosphorylation; Membrane protein; Axin; Rat; Enzyme; Transferases; Rodentia; Central nervous system; Glutamate receptor; synaptic scaffolding molecule; Subunit; Encephalon; β-catenin; Vertebrata; Synapse; Mammalia; Neuron; Guanylate kinase; Synaptic transmission; Glycogen synthase kinase-3β; glycogen synthase kinase; NMDA receptor; Catenin
• ISSN: 0022-3042; 1471-4159
• DOI: 10.1111/j.1471-4159.2004.02497.x

Synaptic scaffolding molecule (S‐SCAM) is a synaptic protein that consists of PDZ domains, a guanylate kinase domain, and WW domains. It interacts with N‐methyl‐d‐aspartate receptor subunits, neuroligin, and β‐catenin. Here, we identified Axin as a novel binding partner of S‐SCAM. Axin was co‐immunoprecipitated with S‐SCAM from rat brain, detected in the post‐synaptic density fraction in rat brain subcellular fractionation, and partially co‐localized with S‐SCAM in neurons. The guanylate kinase domain of S‐SCAM directly bound to the GSK3β‐binding region of Axin. S‐SCAM formed a complex with β‐catenin and Axin, but competed with GSK3β for Axin‐binding. Thereby, S‐SCAM inhibited the Axin‐mediated phosphorylation of β‐catenin by GSK3β.