Identification of Intestinal Fungal Microflora and Bacterial Pathogens in the Collected Adult Ixodes ricinus from the Northern Provinces of Iran

• Published in: Journal of Arthropod-Borne Diseases Vol. 16; no. 2; pp. 97 - 107
• Main Authors: Yousefi-Behzadi, Manijeh, Moazzezy, Neda, Rohani, Mahdi, Naddaf, Saied Reza, Mostafavi, Ehsan, Mohamadi, Ali, Shams-Ghahfarokhi, Masoomeh, Pashootan, Nasrin, Razzaghi-Abyaneh, Mehdi
• Format: Journal Article
• Language: English
• Published: Iran Tehran University of Medical Sciences 01-06-2022
• Subjects: Animals; Arachnids; Arthropods; Bacteria; Cattle; Fever; Fungal species; Fungi; Homogenization; Identification; Infections; Ixodes ricinus; Lyme disease; Microbiome; Microbiota; Microflora; Microorganisms; Morphology; Mycoflora; Original; Parasitic diseases; Pathogens; Provinces; Sheep; Zoonoses; Iran; Caspian Sea; Europe; Microflora; Ixodes ricinus; Mycoflora; Fungal species; Microbiome
• ISSN: 2322-1984; 1735-7179; 2322-2271
• DOI: 10.18502/jad.v16i2.11801

Ticks are vectors of many pathogens that involve various important diseases in humans and animals, they have several diverse hosts consequently can retain a diverse group of indigenous microbes, from bacteria to fungi. Little is known about the prevalence and diversity of tick microflora colonizing the midgut and their effects on ticks and their interaction. This information is important for development of vector control strategies.
This study was carried out in northern Iran during autumn 2019. Ticks,
caught alive on the bodies of domestic animals in the fall. The tick homogenate was prepared. The identification of fungal isolates was carried out according to a combination of macro and microscopic morphology and molecular sequencing. Pathogenic bacteria of the family Borreliaceae,
,
and
were tested by real-time PCR.
A total of 133 mature
ticks were collected from domestic animals, including 71.5% cattle and 28.5% sheep. The tick frequency rates were 87.21% for Mazandaran, 8.28% for Golestan and 4.51% for Gilan Provinces. Total prevalence of fungal tick contamination was 53.4% (75/133) of which
(57%) was the most prevalent species followed by
spp. (42%),
(19%) and
(14%). All tick samples were negative for three pathogenic bacteria including
,
, and
by real-time PCR analysis.
These results show a first picture of the microbial diversity of ticks and highlight the importance of microbiota and their role in host-pathogen interaction.