Overexpression of EMS1/cortactin in NIH3T3 fibroblasts causes increased cell motility and invasion in vitro

• Published in: Oncogene Vol. 16; no. 25; pp. 3227 - 3232
• Main Authors: PATEL, A. S, SCHECHTER, G. L, WASILENKO, W. J, SOMERS, K. D
• Format: Journal Article
• Language: English
• Published: Basingstoke Nature Publishing 25-06-1998; Nature Publishing Group
• Subjects: 3T3 Cells - cytology; 3T3 Cells - metabolism; Animals; Biological and medical sciences; Boyden chamber; Breast cancer; Breast carcinoma; Cell Adhesion - physiology; Cell Division - physiology; Cell migration; Cell Movement - physiology; Cell physiology; Cell proliferation; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes; Chromosome 11; Cortactin; Cytoskeleton; DNA, Complementary - genetics; Fibroblasts; Fundamental and applied biological sciences. Psychology; Gene Expression - genetics; Gene mapping; Head & neck cancer; Head and neck carcinoma; Humans; Invasiveness; Kinases; Lymph nodes; Metastases; Mice; Microfilament Proteins - genetics; Microfilament Proteins - physiology; Molecular and cellular biology; Motility; Neoplasm Proteins - genetics; Neoplasm Proteins - physiology; Phenotypes; Recombinant Proteins - genetics; Signal transduction; Squamous cell carcinoma; Tumors; Squamous cell carcinoma; Motility; Rodentia; Gene overexpression; Metastasis; Gene expression; Adhesion; In vitro; Cell motility; Vertebrata; Mammalia; Cell line; Gene; Mouse; Tumor progression; Fibroblast
• ISSN: 0950-9232; 1476-5594
• DOI: 10.1038/sj.onc.1201850

Cortactin, a p80/85 protein first identified as a src kinase substrate, is thought to be involved in the signaling pathway of mitogenic receptors and adhesion molecules mediating cytoskeletal reorganization. The cortactin gene, EMS1, maps to chromosome 11q13, a region amplified in head and neck squamous cell carcinomas (HNSCC) and breast cancer, which display lymph node metastasis and an unfavorable clinical outcome. To further address the role of cortactin in the malignant phenotype of cells, we stably overexpressed cortactin in NIH3T3 fibroblasts and evaluated the effects of elevated cortactin on cellular proliferation, motility and invasiveness. Cortactin overexpressing cells did not display any striking morphological changes, nor any significant differences in cell proliferation or saturation density as compared to control NIH3T3 cells. Furthermore, the cortactin overexpressing cells were anchorage dependent for growth. Interestingly, cortactin overexpressing cells were more motile and invasive in modified Boyden chamber assays. These results suggest that overexpression of cortactin may play a role in tumor progression by influencing tumor cell migration and invasion.