The Effect of a Hyaluronan-Carboxymethylcellulose Membrane vs. Polyglactin 910 Mesh on Intra-Abdominal Tumor Formation in Mice

• Published in: Diseases of the colon & rectum Vol. 51; no. 9; pp. 1403 - 1407
• Main Authors: Lee, Peter K., Windsperger, Andrew P., Wilson, Christopher M., McCarthy, James B., Wasiluk, Karen R., Rothenberger, David A., Bullard Dunn, Kelli M.
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01-09-2008; The ASCRS; Springer; Lippincott Williams & Wilkins Ovid Technologies
• Subjects: Adjuvants, Immunologic - pharmacology; Animals; Biological and medical sciences; Carboxymethylcellulose Sodium - pharmacology; Colorectal Surgery; Female; Gastroenterology; Gastroenterology. Liver. Pancreas. Abdomen; Hyaluronic Acid - pharmacology; Immunocompromised Host; Medical sciences; Medicine; Medicine & Public Health; Membranes, Artificial; Mice; Mice, SCID; Models, Animal; Neoplasm Seeding; Oncology; Original Contribution; Peritoneal Neoplasms - pathology; Polyglactin 910 - pharmacology; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Surgery; Surgical Mesh; Surgical Oncology; Tumor Cells, Cultured; Tumors; Hyaluronan; Polyglactin; Vicryl; Colon cancer; Seprafilm; Cellulose(carboxymethyl); Rodentia; Mesh; Malignant tumor; Abdomen; Colonic disease; Vertebrata; Mammalia; Mouse; Animal; Gastroenterology; Digestive diseases; Intestinal disease; Cancer
• ISSN: 0012-3706; 1530-0358
• DOI: 10.1007/s10350-008-9299-z

Purpose
Hyaluronan mediates growth of SW620 colon cancer cells
.
Because hyaluronan is the active ingredient in Seprafilm®, we hypothesized that Seprafilm® would affect intraperitoneal tumor growth in a mouse model of peritoneal seeding.
Methods
Immunodeficient mice underwent laparotomy and intraperitoneal inoculation of 10
5
SW620 cells. Seprafilm® (n = 22), Vicryl mesh (foreign body control; n = 24), or no material (sham; n = 19) was placed under the incision. Mice were killed after four weeks and tumors were dissected, counted, and weighed.
Results
Ninety-five percent of mice in the sham group and 96 percent in the Vicryl group developed intraperitoneal tumors. In contrast, only 64 percent of mice in the Seprafilm® group developed tumors (
P
 = 0.024), and these tumors were smaller than those in the sham group; (Seprafilm® = 42 ± 9 mg
vs.
sham = 82 ± 17 mg;
P
 = 0.05). In contrast, tumors in the Vicryl group were dramatically larger (349 ± 49 mg;
P
 < 0.001
vs.
sham or Seprafilm®).
Conclusions
Despite previous data that suggested that hyaluronan increases colon cancer cell growth, we found that Seprafilm® decreased tumor formation and tended to decrease size in this model. In contrast, Vicryl mesh increased tumor formation and size. Our results suggest that Seprafilm® does not promote intraperitoneal tumor growth, especially compared with Vicryl mesh.