WRR4B contributes to a broad‐spectrum disease resistance against powdery mildew in Arabidopsis

• Published in: Molecular plant pathology Vol. 25; no. 1; pp. e13415 - n/a
• Main Authors: Mei, Shuangshuang, Song, Yuxin, Zhang, Zuer, Cui, Haitao, Hou, Shuguo, Miao, Weiguo, Rong, Wei
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01-01-2024; John Wiley and Sons Inc
• Subjects: Airborne microorganisms; Arabidopsis; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Biosynthesis; broad‐spectrum resistance; Calcium-binding protein; Calmodulin; Cell death; Disease resistance; Disease Resistance - genetics; Erysiphe - metabolism; Feedback loops; Flowers & plants; Fungi; Gene expression; Genes; Genetic engineering; Hypersensitive response; Infections; Inoculation; Interleukin 1; Isochorismate synthase; Leucine; Nucleotides; Original; Pathogenesis; Pathogens; Plant Diseases - genetics; Plant resistance; Positive feedback; Powdery mildew; Proteins; Quantitative analysis; Rubber trees; Salicylic acid; Salicylic Acid - metabolism; Signal Transduction; White rust; WHITE RUST RESISTANCE 4B; broad-spectrum resistance; powdery mildew; Arabidopsis; WHITE RUST RESISTANCE 4B
• ISSN: 1464-6722; 1364-3703
• DOI: 10.1111/mpp.13415

Oidium heveae HN1106, a powdery mildew (PM) that infects rubber trees, has been found to trigger disease resistance in Arabidopsis thaliana through ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1)‐, PHYTOALEXIN DEFICIENT 4 (PAD4)‐ and salicylic acid (SA)‐mediated signalling pathways. In this study, a typical TOLL‐INTERLEUKIN 1 RECEPTOR, NUCLEOTIDE‐BINDING, LEUCINE‐RICH REPEAT (TIR‐NB‐LRR)‐encoding gene, WHITE RUST RESISTANCE 4 (WRR4B), was identified to be required for the resistance against O. heveae in Arabidopsis. The expression of WRR4B was upregulated by O. heveae inoculation, and WRR4B positively regulated the expression of genes involved in SA biosynthesis, such as EDS1, PAD4, ICS1 (ISOCHORISMATE SYNTHASE 1), SARD1 (SYSTEMIC‐ACQUIRED RESISTANCE DEFICIENT 1) and CBP60g (CALMODULIN‐BINDING PROTEIN 60 G). Furthermore, WRR4B triggered self‐amplification, suggesting that WRR4B mediated plant resistance through taking part in the SA‐based positive feedback loop. In addition, WRR4B induced an EDS1‐dependent hypersensitive response in Nicotiana benthamiana and contributed to disease resistance against three other PM species: Podosphaera xanthii, Erysiphe quercicola and Erysiphe neolycopersici, indicating that WRR4B is a broad‐spectrum disease resistance gene against PMs.
WRR4B mediates broad‐spectrum disease resistance against powdery mildews Oidium heveae, Podosphaera xanthii, Erysiphe quercicola and E. neolycopersici through taking part in a salicylic acid‐based positive feedback loop.