The Metalloproteinase adam19b Is Required for Sensory Axon Guidance in the Hindbrain

• Published in: Frontiers in neural circuits Vol. 13; p. 14
• Main Authors: Cox, Jane A, Voigt, Mark M
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Research Foundation 06-03-2019; Frontiers Media S.A
• Subjects: Axon guidance; Circuits; Cloning; cranial nerves; CRISPR; Danio rerio; Ganglia; Genes; Genetic screening; Green fluorescent protein; Hindbrain; metalloprotease; Microscopy; Missense mutation; Mutation; Nervous system; Neuroscience; Neurosciences; Proteins; sensory circuits; Sensory neurons; Skull; Zebrafish; United States > US; cranial nerves; metalloprotease; zebrafish; axon guidance; sensory circuits
• ISSN: 1662-5110; 1662-5110
• DOI: 10.3389/fncir.2019.00014

Little is known about the molecular and cellular mechanisms involved in the formation of the cranial peripheral sensory system in vertebrates. To identify genes involved in the formation of these circuits, we performed a forward genetic screen utilizing a transgenic zebrafish line (
) that expresses green fluorescent protein (gfp) in sensory neurons of the Vth, VIIth, IXth and Xth cranial ganglia. Here, we describe a novel zebrafish mutant in which a missense mutation in the
gene selectively affects the epibranchial sensory circuits.