A glucose biosensor based on chitosan–glucose oxidase–gold nanoparticles biocomposite formed by one-step electrodeposition

• Published in: Analytical biochemistry Vol. 334; no. 2; pp. 284 - 289
• Main Authors: Luo, Xi-Liang, Xu, Jing-Juan, Du, Ying, Chen, Hong-Yuan
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15-11-2004
• Subjects: Biocompatible Materials - chemistry; Biosensing Techniques - methods; Blood Glucose - analysis; Chitosan; Chitosan - chemistry; Electrochemical deposition; Electroplating - methods; Enzymes, Immobilized - chemistry; Enzymes, Immobilized - metabolism; Glucose - analysis; Glucose biosensor; Glucose Oxidase - chemistry; Glucose Oxidase - metabolism; Gold - chemistry; Gold nanoparticles; Hydrogen-Ion Concentration; Immobilization; Nanostructures - chemistry; Reproducibility of Results; Solutions; Spectrum Analysis; Temperature; Time Factors; Electrochemical deposition; Immobilization; Glucose biosensor; Chitosan; Gold nanoparticles
• ISSN: 0003-2697; 1096-0309
• DOI: 10.1016/j.ab.2004.07.005

An amperometric biosensor for the quantitative measurement of glucose is reported. The biosensor is based on a biocomposite that is homogeneous and easily prepared. This biocomposite is made of chitosan hydrogel, glucose oxidase, and gold nanoparticles by a direct and facile electrochemical deposition method under enzyme-friendly conditions. The resulting biocomposite provided a shelter for the enzyme to retain its bioactivity at considerably extreme conditions, and the decorated gold nanoparticles in the biocomposite offer excellent affinity to enzyme. The biosensor exhibited a rapid response (within 7
s) and a linear calibration range from 5.0
μM to 2.4
mM with a detection limit of 2.7
μM for the detection of glucose. The combination of gold nanoparticles affinity and the promising feature of the biocomposite with the onestep nonmanual technique favor the sensitive determination of glucose with improved analytical capabilities.