Regulation of cyanogenic glucosides in wild and domesticated Eusorghum taxa

• Published in: Plant biology (Stuttgart, Germany) Vol. 24; no. 6; pp. 1084 - 1088
• Main Authors: Myrans, H., Gleadow, R. M., Wittstock, U.
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01-10-2022; John Wiley and Sons Inc
• Subjects: Cereal crops; Crop wild relatives; cyanogenesis; cyanogenic glucosides; Domestication; Glucosides; Glycosides - metabolism; Hydrogen cyanide; Hydrogen Cyanide - metabolism; Leaves; Metabolism; Metabolites; Nitrogen - metabolism; Plants - metabolism; Short; Sorghum; Sorghum - metabolism; Speciation; Taxa; sorghum; Crop wild relatives; cyanogenesis; domestication; cyanogenic glucosides
• ISSN: 1435-8603; 1438-8677
• DOI: 10.1111/plb.13447

Domesticated sorghum (Sorghum bicolor [L.] Moench subsp. bicolor) diverts significant amounts of nitrogen away from primary metabolism to the synthesis of cyanogenic glucosides (CNglc) – specialized metabolites that release toxic hydrogen cyanide (HCN). Our aim was to identify the point in the genus Sorghum Moench at which plants gained the ability to maintain hazardous concentrations of cyanogenic glucosides in their leaves into maturity (HCN potential >0.4 mg g−1). This ability occurs in domesticated sorghum (in the subgenus Eusorghum), but not in wild taxa in other Sorghum subgenera.
Eight accessions from the subgenus Eusorghum were grown in a common garden: an improved sorghum line, five sorghum landraces, the crop's wild progenitor (S. bicolor subsp. verticilliflorum [Steud.] de Wet ex Wiersema & J. Dahlb.) and wild Sorghum propinquum (Kunth) Hitchc. HCN potential was measured in plants (n = 80) at the three‐leaf stage and at 6 weeks old.
All study accessions, including the wild taxa, had hazardous CNglc concentrations in the leaves at both the three‐leaf stage (mean HCN potentials > = 2.5 mg g−1) and at 6 weeks old (mean HCN potentials > = 0.68 mg g−1), greatly contrasting the much lower mature leaf HCN potentials previously found in wild Sorghum taxa outside subgenus Eusorghum (generally <= 0.01 mg g−1).
Our results suggest that the ability to maintain hazardous leaf HCN potentials into maturity might have arisen during the divergence of Eusorghum from other Sorghum subgenera, rather than during the speciation or domestication of S. bicolor, and highlights the value of utilizing Sorghum taxa outside Eusorghum in efforts to improve the crop safety of sorghum.
This study presents evidence that the ability to maintain high leaf cyanogenic glucoside concentrations into maturity might have arisen in sorghum's crop wild relatives, rather than during domestication of the crop.