Rbm14 maintains the integrity of genomic DNA during early mouse embryogenesis via mediating alternative splicing

• Published in: Cell proliferation Vol. 53; no. 1; pp. e12724 - n/a
• Main Authors: Li, Jing, Wang, Chenxin, Feng, Guihai, Zhang, Linlin, Chen, Guilai, Sun, Hao, Wang, Jiaqiang, Zhang, Ying, Zhou, Qi, Li, Wei
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01-01-2020; John Wiley and Sons Inc
• Subjects: Alternative Splicing; Animals; Antibodies; Apoptosis; Bioassays; Cell growth; Cell proliferation; Comet assay; CRISPR; Damage accumulation; Damage detection; Defects; Deoxyribonucleic acid; DNA; DNA Damage; Embryo cells; Embryogenesis; Embryonic Development; Embryonic growth stage; Embryos; Ethanol; Female; Flow cytometry; Gastrulation; Genes; Genomes; Genomic Instability; Immunoprecipitation; Implantation; Integrity; Laboratory animals; Lethality; Maintenance; Male; Mass spectra; Mice; Mice, Inbred ICR; Mice, Knockout; Microinjection; Mouse Embryonic Stem Cells - metabolism; mRNA; Original; Proteins; Ribonucleic acid; RNA; Rodents; Splicing; Stem cell transplantation; Stem cells; Transcription Factors - genetics; Transcription Factors - metabolism; Zoology; Beijing China; China
• ISSN: 0960-7722; 1365-2184
• DOI: 10.1111/cpr.12724

Objective
In this study, we generated an Rbm14 knockout mouse model to explore its functions during early mouse embryogenesis.
Materials and methods
The Rbm14 knockout mouse model was generated by a combination of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 and microinjection techniques. The developmental defects of the knockout embryos were characterized by histological analyses. The accumulation of DNA damage in mouse embryonic stem cells (ESCs) was detected by γH2AX staining and comet assay. The altered mRNA splicing of DNA damage response (DDR)‐related genes was detected by RNA‐Seq analysis and confirmed by semi‐quantitative PCR. The interaction of RBM14 with alternative splicing‐related genes was detected by immunoprecipitation‐mass spectra (IP‐MS) and confirmed by co‐immunoprecipitation (Co‐IP).
Results
Rbm14 knockout in mice results in apoptosis and cell proliferation defects in early post‐implantation epiblast cells, leading to gastrulation disruption and embryonic lethality. FACS and immunostaining demonstrate accumulation of DNA damage in Rbm14 knockout ES cells. We also identified altered splicing of DDR‐related genes in the knockout mouse ESCs by RNA‐Seq, indicating that RBM14‐mediated alternative splicing is required for the maintenance of genome integrity during early mouse embryogenesis.
Conclusions
Our work reveals that Rbm14 plays an essential role in the maintenance of genome integrity during early mouse embryonic development by regulating alternative splicing of DDR‐related genes.