Monitoring of diffusion properties and transverse relaxation time of mouse ischaemic muscle after administration of human mesenchymal stromal cells derived from adipose tissue

• Published in: Cell proliferation Vol. 52; no. 6; pp. e12672 - n/a
• Main Authors: Skorupa, Agnieszka, Ciszek, Mateusz, Pilny, Ewelina, Smolarczyk, Ryszard, Jarosz–Biej, Magdalena, Boguszewicz, Łukasz, Krakowczyk, Łukasz, Szala, Stanisław, Sokół, Maria, Cichoń, Tomasz
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01-11-2019; John Wiley and Sons Inc
• Subjects: Adipose tissue; Adipose Tissue - cytology; Animal tissues; Animals; Body fat; Cell Differentiation - drug effects; Cell Differentiation - physiology; Cells, Cultured; diffusion; Diffusion Tensor Imaging - methods; Eigenvalues; Femoral artery; Fibers; Fibroblasts; Fibroblasts - pathology; Humans; Immunocompetence; Injection; ischaemia; Ischemia; Ischemia - pathology; Macrophages; Magnetic resonance imaging; Male; Mathematical analysis; Medical imaging; Mesenchymal stem cells; Mesenchymal Stem Cells - cytology; mesenchymal stromal cells; Mice, Transgenic; Monitoring; Muscle contraction; Muscles; Neovascularization, Physiologic - physiology; Original; peripheral artery disease; Regeneration - drug effects; Regeneration - physiology; Relaxation time; Stromal cells; Tensors; Transplantation; Transplants & implants; Vascular diseases; Veins & arteries; diffusion; ischaemia; magnetic resonance imaging; peripheral artery disease; mesenchymal stromal cells; transplantation
• ISSN: 0960-7722; 1365-2184
• DOI: 10.1111/cpr.12672

Objectives
Application of non‐invasive imaging methods plays an important role in the assessment of cellular therapy effects in peripheral artery disease. The purpose of this work was to evaluate the kinetics of MRI‐derived parameters characterizing ischaemic hindlimb muscle after administration of human mesenchymal stromal cells derived from adipose tissue (hADSC) in mice.
Materials and methods
MRI experiments were performed on a 9.4T Bruker system. The measurement protocol included transverse relaxation time mapping and diffusion tensor imaging. The monitoring period encompassed 14 days after femoral artery ligation and subsequent cell administration. The effect of hADSC transplantation was compared with the effect of normal human dermal fibroblasts (NHDFs) and phosphate‐buffered saline injection.
Results
The most significant differences between the hADSC group and the remaining ones were observed around day 3 after ischaemia induction (increased transverse relaxation time in the hADSC group in comparison with the control group) and around day 7 (increased transverse relaxation time and decreased third eigenvalue of the diffusion tensor in the hADSC group in comparison with the control and NHDF groups) at the site of hADSC injection. Histologically, it was associated with increased macrophage infiltration at days 3‐7 and with the presence of small regenerating fibres in the ischaemic tissue at day 7.
Conclusions
Our results underscore the important role of macrophages in mediating the therapeutic effects of hADSCs and confirm the huge potential of magnetic resonance imaging in monitoring of cellular therapy effects.