Andrimid production at low temperature by a psychrotolerant Serratia proteamaculans strain

• Published in: World journal of microbiology & biotechnology Vol. 29; no. 10; pp. 1773 - 1781
• Main Authors: Sánchez, Leandro A., Sierra, Manuel González, Siñeriz, Faustino, Delgado, Osvaldo
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-10-2013; Springer Nature B.V
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - metabolism; Antibiotics; Antimicrobial agents; Applied Microbiology; Bacteria; Biochemistry; Biomedical and Life Sciences; Bioreactors; Bioreactors - microbiology; Biotechnology; Chromatography, Liquid; Cold; Cold Temperature; E coli; Environmental Engineering/Biotechnology; Enzymes; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - ultrastructure; Life Sciences; Low temperature; Magnetic Resonance Spectroscopy; Microbial Sensitivity Tests; Microbiology; Microorganisms; Microscopy, Electron; Original Paper; Pathogens; Peptides; Pharmaceutical industry; Polyenes - chemistry; Polyenes - isolation & purification; Polyenes - metabolism; Pyrroles - chemistry; Pyrroles - isolation & purification; Pyrroles - metabolism; Salmonella; Serratia - growth & development; Serratia - metabolism; Serratia proteamaculans; Studies; Tandem Mass Spectrometry; Temperature; Temperature effects; United States > US; Antimicrobial; Serratia proteamaculans; Psychrotolerant; Andrimid; Pseudo-peptide
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-013-1338-9

Andrimid, a known non-ribosomal pseudo-peptide antibiotic, was isolated from a psychrotolerant
Serratia proteamaculans
strain. The antibiotic peptide was produced at low temperature (8 °C) in a 7.5 l BIOFLO 101 bioreactor under batch culture mode. Andrimid activity from
S. proteamaculans
culture was only detected at 25 °C and below and potent antibacterial activity was revealed against both, pathogenic and non-pathogenic bacteria. Minimal inhibitory concentration values determined by microdilution experiments varied in the range between 0.01 and 0.78 μg/ml. Antimicrobial purification and structure elucidation were carried out by LC-MS/MS and
1
H/
13
C NMR approaches. The effects on the ultrastructure of sensitive
Escherichia coli
35,218 cells were observed by transmission electron microscopy at different inhibition stages. This work demonstrated the significance of bioprospection from cold environments through the screening of microorganisms with ability to produce cold-active biomolecules of biotechnological interest.
S. proteamaculans
136 was revealed as a novel microbial source for andrimid production at low temperatures, showing biotechnological potential to be applied in cryopreservation, food or cosmetic industries against pathogenic bacteria.