Activity-dependent fluorescent labeling of bacterial cells expressing the TOL pathway

• Published in: Journal of microbiological methods Vol. 60; no. 1; pp. 41 - 46
• Main Authors: Clingenpeel, Scott R., Keener, William K., Keller, Caron R., De Jesus, Karl, Howard, M. Hope, Watwood, Mary E.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier B.V 2005; Elsevier Science
• Subjects: 3-Ethynylbenzoate; AROMATICS; Bacteriology; Benzoates - metabolism; Biological and medical sciences; Bioremediation; Chromogenic; ENVIRONMENTAL SCIENCES, 60 - APPLIED LIFE SCIENCES; ENZYMES; FISSION; Fluorescent Dyes - metabolism; Fluorogenic; Fundamental and applied biological sciences. Psychology; Microbiology; Microscopy, Fluorescence; Miscellaneous; Oxygenases - metabolism; PROBES; PSEUDOMONAS; Pseudomonas putida - enzymology; Pseudomonas putida - metabolism; PYROCATECHOL; TOLUENE; Toluene - metabolism; Bioremediation; 3-Ethynylbenzoate; Fluorogenic; Toluene; Chromogenic; Enzyme; Bacteria; Pyrocatechol; Strain
• ISSN: 0167-7012; 1872-8359
• DOI: 10.1016/j.mimet.2004.08.018

3-Ethynylbenzoate (3EB) functions as a novel, activity-dependent, fluorogenic, and chromogenic probe for bacterial strains expressing the TOL pathway, which degrade toluene via conversion to benzoate, followed by
meta ring fission of the intermediate catechol. This direct physiological analysis allows the fluorescent labeling of cells whose toluene-degrading enzymes have been induced by an aromatic substrate.