Actin-depolymerizing factor from Eimeria tenella promotes immunogenic function of chicken dendritic cells

• Published in: Parasitology research (1987) Vol. 120; no. 2; pp. 579 - 592
• Main Authors: Lakho, Shakeel Ahmed, Haseeb, Muhammad, Huang, Jianmei, Yang, Zhang, Hasan, Muhammad Waqqas, Aleem, Muhammad Tahir, Memon, Muhammad Ali, Song, XiaoKai, Yan, RuoFeng, Xu, Lixin, Li, XiangRui
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-02-2021; Springer; Springer Nature B.V
• Subjects: Actin; Actin-depolymerizing protein; Analysis; Animals; Antigens; Biomedical and Life Sciences; Biomedicine; CCR7 protein; CD11c antigen; CD3 antigen; CD4 antigen; CD80 antigen; CD83 antigen; CD86 antigen; Cell Differentiation; Cell Proliferation; Chickens; Coccidiosis - immunology; Coccidiosis - parasitology; Coccidiosis - prevention & control; Cytokines - immunology; Dendritic cells; Dendritic Cells - immunology; Destrin - genetics; Destrin - metabolism; Eimeria tenella; Eimeria tenella - genetics; Eimeria tenella - immunology; Flow cytometry; Host-parasite interactions; Humans; Immune response; Immunization; Immunofluorescence; Immunogenicity; Immunology; Immunology and Host-Parasite Interactions - Original Paper; Interleukin 10; Interleukin 12; Interleukin 4; Lymphocyte Activation; Lymphocytes; Lymphocytes T; Medical Microbiology; Microbiology; Muscle proteins; Phenotypes; Protozoan Proteins - genetics; Protozoan Proteins - metabolism; Rats; Spleen - immunology; T cells; Th1 Cells - immunology; Transcription; Transforming growth factors; γ-Interferon; China; Actin-depolymerizing factor; CD4; Dendritic cells; Cytokines; species; Toll-like receptors; Avian; T cells; Avian Eimeria species; CD4+ T cells
• ISSN: 0932-0113; 1432-1955
• DOI: 10.1007/s00436-020-07016-4

Dendritic cells play a crucial role in inducing antigen-specific immunity to pathogens. During host-parasite interaction, host immune response to the parasite molecules is considered essential for recognizing novel antigens for control strategies. Therefore, in the present study, chicken dendritic cells (DCs) (ChDCs), derived from spleens were used to evaluate their capacity to proliferate and differentiate autologous T lymphocytes in response to actin-depolymerizing factor from
Eimeria tenella
(EtADF). Immunoblot analysis showed that recombinant EtADF protein (rEtADF) was able to interact with rat anti-rEtADF antibodies. The immunofluorescence test confirmed rEtADF binding on ChDCs surface. Flow cytometric analysis revealed that phenotypes for MHCII, CD1.1, CD11c, CD80, and CD86 were increased in ChDCs after rEtADF treatment. qRT-PCR results indicated that ChDCs triggered TLR signaling in response to rEtADF, and suppressed Wnt signaling. Transcript levels of CD83, CCL5, and CCR7 in ChDCs were improved following rEtADF treatment. In addition, rEtADF promoted DC-directed T cell proliferation and differentiation of naïve T cells into CD3
+
/CD4
+
T cells in DC/T cell co-incubation system. Cytokine analysis of rEtADF-pulsed ChDCs showed increased levels of IL-12 and IFN-γ, while IL-10 and TGF-β remained unchanged. Moreover, rEtADF-treated ChDCs enhanced production of IFN-γ when incubated with T cells, and IL-4 secretion remained unchanged. Our findings indicted that rEtADF could facilitate the polarization of Th1 immune cells by triggering both host DCs and T cells. Our findings provide useful insights into future work aimed at anticoccidial vaccine strategies.