Ferrocene Branched Chitosan for the Construction of a Reagentless Amperometric Hydrogen Peroxide Biosensor

• Published in: Macromolecular bioscience Vol. 7; no. 4; pp. 435 - 439
• Main Authors: Garcia, Ariel, Peniche-Covas, Carlos, Chico, Belkis, Simpson, Benjamin K., Villalonga, Reynaldo
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 10-04-2007; WILEY‐VCH Verlag; Wiley-VCH
• Subjects: Applied sciences; Biological and medical sciences; Biosensing Techniques; Biosensors; Biotechnology; chitosan; Chitosan - chemistry; Electrodes; Enzymes, Immobilized - chemistry; Exact sciences and technology; Ferrous Compounds - chemistry; Fundamental and applied biological sciences. Psychology; Horseradish Peroxidase - chemistry; Hydrogen Peroxide - analysis; immobilized enzymes; Metallocenes; Methods. Procedures. Technologies; Natural polymers; Physicochemistry of polymers; polysaccharides; Starch and polysaccharides; Various methods and equipments; Condensation reaction; Hydrogen peroxide; Support; Enzyme; Organometallic polymer; Experimental study; Redox polymer; Metallocene; Chemical modification; Sensitivity; Peroxidases; Chitosan derivatives; Ferrocene derivative polymer; Storage stability; Biosensor; Preparation; Detection threshold; Oside polymer; Peroxidase; Oxidoreductases; Chitosan; Immobilized enzyme
• ISSN: 1616-5187; 1616-5195
• DOI: 10.1002/mabi.200700032

Chitosan was chemically branched with ferrocene moieties and further used as a support for the immobilization of horseradish peroxidase on a glassy carbon electrode. The reagentless biosensor device showed a linear amperometric response toward hydrogen peroxide concentrations between 35 × 10−6 M and 2.0 × 10−3 M. The biosensor reached 95% of the steady‐state current in about 20 s and its sensitivity was 28.4 × 10−3 µA · M−1. The enzyme electrode retained 94% of its initial activity after 2 weeks of storage at 4 °C in 50 × 10−3 M sodium phosphate buffer at pH 7.0.