Optical-Spectrometry-Based Method for Immunosuppressant Medicine Level Detection in Aqueous Solutions

• Published in: Sensors (Basel, Switzerland) Vol. 18; no. 7; p. 2001
• Main Authors: Marzejon, Marcin, Kosowska, Monika, Majchrowicz, Daria, Bułło-Piontecka, Barbara, Wąsowicz, Michał, Jędrzejewska-Szczerska, Małgorzata
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 22-06-2018; MDPI AG
• Subjects: cyclosporine; label-free detection; optical spectrophotometry; cyclosporine; label-free detection; optical spectrophotometry
• ISSN: 1424-8220; 1424-8220
• DOI: 10.3390/s18072001

In this paper, an investigation into detecting immunosuppressive medicine in aqueous solutions using a spectrometry-based technique is described. Using optical transmissive spectrometry, absorbance measurements in the spectra range from 250 nm to 1000 nm were carried out for different cyclosporine A (CsA) concentrations in aqueous solutions. The experiment was conducted for samples both with and without interferent substances-glucose and sodium chloride. Using a dedicated algorithm, the measured data was analyzed and a high correlation coefficient R² = 0.8647 was achieved. The experiment showed that the described technique allowed for the detection of various CsA concentration levels in a selective, label-free and simple way. This method could be used in medicine, veterinary medicine and laboratory diagnostics.