Linking traditional anti-ulcer use of rhizomes of Bergenia ciliata (Haw.) to its anti-Helicobacter pylori constituents

• Published in: Natural product research Vol. 34; no. 4; pp. 541 - 544
• Main Authors: Ali, Ejaz, Arshad, Najma, Bukhari, Nadeem Irfan, Nawaz Tahir, Muhammad, Zafar, Shamail, Hussain, Amjad, Parveen, Sajida, Qamar, Shaista, Shehzadi, Naureen, Hussain, Khalid
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 16-02-2020; Taylor & Francis Ltd
• Subjects: Amoxicillin; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; anti-Helicobacter pylori; anti-ulcer; Aquatic plants; Asia; Benzopyrans - isolation & purification; Benzopyrans - therapeutic use; Bergenia ciliata; bergenin; Bioassays; Constituents; Crystallization; Dilution; Helicobacter pylori; Helicobacter pylori - drug effects; Humans; Medicine, Traditional; Methanol; Microbial Sensitivity Tests; Minimum inhibitory concentration; Plant Extracts - chemistry; Plant Extracts - pharmacology; Rhizome; Rhizomes; Saxifragaceae; Saxifragaceae - chemistry; Stomach Ulcer - drug therapy; Ulcer - drug therapy; Ulcers; Asia; anti-ulcer; Bergenia ciliata; bergenin; Saxifragaceae; anti-Helicobacter pylori
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2018.1488711

The rhizomes of Bergenia ciliata (B. ciliata, Family: Saxifragaceae) are widely used for treating gastric ulcers in folk medicine in Asia. It was hypothesized that anti-ulcer activity of B. ciliata is due to its anti-Helicobacter pylori (H. pylori) activity. The anti-H. pylori activity was investigated on six clinical bacterial isolates using agar well-diffusion and broth micro-dilution methods. The anti-H. pylori activity of amoxicillin (standard) was the highest (Zone of inhibition; ZI = 25 mm, minimum inhibitory concentration; MIC=0.125 µg/µL) whereas among all the extracts of the rhizomes, methanol extract showed the highest activity (ZI = 16 mm, MIC = 12.50 µg/µL). Bioassay guided isolation of methanol extract using chromatographic and crystallization techniques isolated bergenin (ZI = 21mm, MIC = 0.391µg/µL) as constituent responsible for anti-H. pylori activity. The present study describes for the first time anti-H. pylori activity and possible mechanism of anti-ulcer properties of rhizomes of B. ciliata.