Development of phyllanthin containing microcapsules and their improved biological activity towards skin cells and Staphylococcus aureus

• Published in: Bioorganic & medicinal chemistry letters Vol. 22; no. 1; pp. 468 - 471
• Main Authors: Lam, Pik-Ling, Gambari, Roberto, Yip, Joanne, Yuen, Marcus Chun-Wah, Lam, Kim-Hung, Wong, Raymond Siu-Ming, Wang, Xiao-Wen, Tang, Johnny Cheuk-On, Kok, Stanton Hon-Lung, Chui, Chung-Hin
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 01-01-2012; Elsevier
• Subjects: Anti-Bacterial Agents - pharmacology; Antioxidants - chemistry; bioactive properties; bioassays; Biological and medical sciences; Capsules - chemistry; chemistry; Chemistry, Pharmaceutical - methods; Chitosan; Chitosan - chemistry; Drug Design; fibroblasts; Fibroblasts - metabolism; Fourier transform infrared spectroscopy; Human skin cells; Humans; keratinocytes; Keratinocytes - metabolism; Lignans - chemistry; Medical sciences; Microcapsule; Microscopy, Electron, Scanning - methods; Models, Chemical; Pharmacology. Drug treatments; Phyllanthin; Reactive Oxygen Species; scanning electron microscopy; Spectroscopy, Fourier Transform Infrared - methods; Staphylococcal Infections - drug therapy; Staphylococcus aureus; Staphylococcus aureus - metabolism; Surface Properties; Time Factors; Chitosan; Human skin cells; Staphylococcus aureus; Microcapsule; Phyllanthin; Human; Polymer; In vitro; Biological activity; Bacteria; Micrococcales; Microparticle; Micrococcaceae; Skin; Polysaccharide
• ISSN: 0960-894X; 1464-3405
• DOI: 10.1016/j.bmcl.2011.10.097

Chitosan based microcapsule which encapsulated with phyllanthin was developed by simple coacervation. The composition and surface morphology of phyllanthin containing microcapsules were analyzed by Fourier Transform Infrared spectroscopy and Scanning Electron Microscopy, respectively. The release of phyllanthin from the microcapsules was found to be more than 60% after 120h. In vitro biological assays demonstrated that these phyllanthin containing microcapsules showed a stronger anti-oxidation potential on both human fibroblasts and keratinocytes as well as a better growth inhibitory activity towards Staphylococcus aureus.
Chitosan based microcapsule which encapsulated with phyllanthin was developed by simple coacervation. The composition and surface morphology of phyllanthin containing microcapsules were analyzed by Fourier Transform Infrared spectroscopy and Scanning Electron Microscopy, respectively. The release of phyllanthin from the microcapsules was found to be more than 60% after 120h. In vitro biological assays demonstrated that these phyllanthin containing microcapsules showed a stronger anti-oxidation potential on both human fibroblasts and keratinocytes as well as a better growth inhibitory activity towards Staphylococcus aureus.