Expression of platelet-derived growth factor-BB in liver tissues of patients with chronic hepatitis B

• Published in: World journal of gastroenterology : WJG Vol. 10; no. 3; pp. 385 - 388
• Main Authors: Lou, Song-Mei, Li, You-Ming, Wang, Kai-Ming, Cai, Wei-Min, Weng, Hong-Lei
• Format: Journal Article
• Language: English
• Published: United States Department of Gastroenterology, Hangzhou First People's Hospital, Hangzhou 310006, Zhejiang Province, China%First Affiliated Hospital, College of Medicine, Zhejiang Univereity,Hangzhou 310003, Zhejiang Province, China 01-02-2004; Baishideng Publishing Group Inc
• Subjects: Becaplermin; Biopsy; Hepatitis B, Chronic - metabolism; Hepatitis B, Chronic - pathology; Humans; Liver - metabolism; Liver - pathology; Liver Cirrhosis - blood; Platelet-Derived Growth Factor - metabolism; Proto-Oncogene Proteins c-sis; Viral Hepatitis; 慢性乙型肝炎; 肝纤维化; 肝组织; 血小板源性生长因子-BB; 血清学检查
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v10.i3.385

AIM:To study the relationship between expression of plateletderived growth factor-BB (PDGF-BB) and fibrogenesis in chronic hepatitis B.METHODS:Hepatic tissues from 43 patients with chronic hepatitis B were embedded in paraffin.The sections were stained with HE and picric acid-sirius red to determine inflammatory activity and fibrosis stages. PDGF-BB expression was detected by immunohistochemistry and assessed semiquantatively. Levels of serum hyaluronic acid (HA),pro-collagen Ⅲ(PCⅢ), collagen Ⅳ (Ⅳ-C) and laminin (LN) were examined by radioimmunoassay (RIA).RESULTS:The expression level of PDGF-BB was found to be positively correlated with inflammatory activity, fibrosis stage and grade of histological findings (τ=0.58,0.55,0.55,P<0.01).The positive correlation was also observed between tissue level of PDGF-BB expression and contents of HA, PCⅢ, Ⅳ-C and LN in the circulation (r=0.52,0.32,0.40,0.33, P<0.05).CONCLUSION: PDGF-BB may play some role in the development and progression of liver fibrosis.