Inflammatory biomarkers on an LPS-induced RAW 264.7 cell model: a systematic review and meta-analysis

• Published in: Inflammation research Vol. 71; no. 7-8; pp. 741 - 758
• Main Authors: Facchin, Bruno Matheus, dos Reis, Gustavo Oliveira, Vieira, Guilherme Nicácio, Mohr, Eduarda Talita Bramorski, da Rosa, Júlia Salvan, Kretzer, Iara Fabricia, Demarchi, Izabel Galhardo, Dalmarco, Eduardo Monguilhott
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01-08-2022; Springer Nature B.V
• Subjects: Allergology; Anti-inflammatory agents; Biomarkers; Biomedical and Life Sciences; Biomedicine; Cell density; Dermatology; Drug development; IL-1β; Immunology; Inflammation; Interleukin 6; Lipopolysaccharides; Meta-analysis; Neurology; Pharmacology/Toxicology; Review; Reviews; Rheumatology; Systematic review; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Tumor necrosis factor-alpha; In vitro techniques; Nitricde; Lipopolysaccharide; Interleukin- 6; Macrophages; Interleukin-1 beta; RAW 264.7 cells
• ISSN: 1023-3830; 1420-908X
• DOI: 10.1007/s00011-022-01584-0

Introduction
Several experimental models have been designed to promote the development of new anti-inflammatory drugs. The in vitro model using RAW 264.7 cells has been widely used. However, there is still no consensus on which inflammatory mediators should initially be measured to screen for possible anti-inflammatory effects. To determine the rationality of measuring inflammatory mediators together with NO, such as the levels of tumor necrosis factor (TNF)-α, and interleukins (IL) 1β and 6, we carried out this systematic review (SR) and meta-analysis (MA).
Methodology
We conducted this SR and MA in accordance with the Preferred Reporting of Systematic Reviews and Meta-Analysis and the Cochrane Handbook for Systematic Reviews of Intervention. This review was registered in the Open Science Framework (
https://doi.org/10.17605/OSF.IO/8C3HT
).
Results
LPS-induced cells produced high NO levels compared to non-LPS induced, and this production was not related to cell density. TNF-α, IL-1β, and IL-6, also showed high levels after cells had been stimulated with LPS. Though with some restrictions, all studies were reliable, as the risk of bias was detected in the test compounds and systems.
Conclusion
Measurement of NO levels may be sufficient to screen for possible anti-inflammatory action in the context of LPS-induced RAW 264.7 cells.