Evaluation of WGA and Concanavalin A (Con A) lectin as biomarkers of hepatosplenic schistosomiasis in human biopsies with no evidence of egg-granuloma system

• Published in: Revista do Instituto de Medicina Tropical de São Paulo Vol. 55; no. 3; pp. 213 - 215
• Main Authors: Rêgo, Moacyr Jesus Barreto de Melo, Vieira-De-Mello, Gabriela Souto, Araújo, Cláudio Witaker, Cavalcanti, Maria do Socorro de Mendonça, Beltrão, Eduardo Isidoro Carneiro
• Format: Journal Article
• Language: English
• Published: Brazil Instituto de Medicina Tropical de Sao Paulo 01-06-2013; Instituto de Medicina Tropical; Universidade de São Paulo (USP)
• Subjects: Biomarkers - analysis; Biopsy; Colon, Sigmoid - chemistry; Colon, Sigmoid - parasitology; Colon, Sigmoid - pathology; Colonic lesions; Concanavalin A - analysis; Humans; Immunohistochemistry; Lectin histochemistry; Schistosomiasis; Schistosomiasis mansoni - metabolism; Schistosomiasis mansoni - pathology; Splenic Diseases - metabolism; Splenic Diseases - parasitology; Splenic Diseases - pathology; TROPICAL MEDICINE; Wheat Germ Agglutinins - analysis; Lectin histochemistry; Schistosomiasis; Colonic lesions
• ISSN: 0036-4665; 1678-9946; 1678-9946; 0036-4665
• DOI: 10.1590/S0036-46652013000300014

Colonic lesions are predominant in patients with schistosomiasis. However, carbohydrate alterations in colonic schistosomiasis remain unclear. Lectin-ligands allow us to identify changes in the saccharide patterns of cells.
Biopsies of descending and rectosigmoid colon of patients were submitted to WGA and Con A lectin histochemistry.
WGA stained stroma and gland cells of descending colon and rectosigmoid tissues in a granular strong cytoplasmatic pattern in schistosomiasis specimens differing from normal control and Con A failing to recognize all samples analyzed.
WGA ligands are expressed differently in patients with hepatosplenic schistosomiasis and no evidence of egg-granuloma system.