tANCHOR fast and cost-effective cell-based immunization approach with focus on the receptor-binding domain of SARS-CoV-2

• Published in: Biology methods and protocols Vol. 8; no. 1; p. bpad030
• Main Authors: Bernauer, Hubert, Schlör, Anja, Maier, Josef, Bannert, Norbert, Hanack, Katja, Ivanusic, Daniel
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 2023
• Subjects: Methods; CD20; tANCHOR; GPCR; SARS-CoV-2; antibodies; immunization
• ISSN: 2396-8923; 2396-8923
• DOI: 10.1093/biomethods/bpad030

Abstract
Successful induction of antibodies in model organisms like mice depends strongly on antigen design and delivery. New antigen designs for immunization are helpful for developing future therapeutic monoclonal antibodies (mAbs). One of the gold standards to induce antibodies in mice is to express and purify the antigen for vaccination. This is especially time-consuming when mAbs are needed rapidly. We closed this gap and used the display technology tetraspanin anchor to develop a reliable immunization technique without the need to purify the antigen. This technique is able to speed up the immunization step enormously and we have demonstrated that we were able to induce antibodies against different proteins with a focus on the receptor-binding domain of SARS-CoV-2 and the extracellular loop of canine cluster of differentiation 20 displayed on the surface of human cells.