Biochemical localization of a protein involved in synthesis of Gluconacetobacter hansenii cellulose

• Published in: Cellulose (London) Vol. 18; no. 3; pp. 739 - 747
• Main Authors: Iyer, Prashanti R., Catchmark, Jeffrey, Brown, Nicole R., Tien, Ming
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-06-2011; Springer Nature B.V; Springer
• Subjects: Acetobacter; Antibodies; biofuels (including algae and biomass), bio-inspired, membrane, carbon sequestration, materials and chemistry by design, synthesis (self-assembly); Bioorganic Chemistry; Cellulose; Ceramics; Chemistry; Chemistry and Materials Science; Composites; E coli; Escherichia coli; Fractionation; Glass; Gluconacetobacter; Histidine; Localization; Natural Materials; Organic Chemistry; Physical Chemistry; Polymer Sciences; Proteins; Sustainable Development; Cellulose synthesis; Periplasm; Gluconacetobacter; AcsD
• ISSN: 0969-0239; 1572-882X
• DOI: 10.1007/s10570-011-9504-4

Using subcellular fractionation and Western blot methods, we have shown that AcsD, one of the proteins encoded by the Acetobacter cellulose synthase (acs) operon, is localized in the periplasmic region of the cell. AcsD protein was heterologously expressed in
Escherichia coli
and purified using histidine tag affinity methods. The purified protein was used to obtain rabbit polyclonal antibodies. The purity of the subcellular fractions was assessed by marker enzyme assays.