Sequence and characterization of the met-7 gene of Neurospora crassa

The proteins encoded by the met-7+ and met-3+ genes of Neurospora crassa are required to form a functional cystathionine-gamma-synthase (CGS). The met-7+ gene has been cloned by complementation of a met-7 mutant. The nucleotide sequence of the complementing DNA reveals the presence of a 542-amino ac...

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Bibliographic Details
Published in:	Gene Vol. 111; no. 2; p. 265
Main Authors:	Crawford, J M, Geever, R F, Asch, D K, Case, M E
Format:	Journal Article
Language:	English
Published:	Netherlands 15-02-1992
Subjects:	Amino Acid Sequence Base Sequence Blotting, Northern Carbon-Oxygen Lyases Cloning, Molecular Genetic Complementation Test Lyases - genetics Methionine - genetics Molecular Sequence Data Neurospora crassa - enzymology Neurospora crassa - genetics
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Summary:	The proteins encoded by the met-7+ and met-3+ genes of Neurospora crassa are required to form a functional cystathionine-gamma-synthase (CGS). The met-7+ gene has been cloned by complementation of a met-7 mutant. The nucleotide sequence of the complementing DNA reveals the presence of a 542-amino acid open reading frame (ORF). Disruption of this ORF abolishes complementation of the met-7 mutation.
ISSN:	0378-1119
DOI:	10.1016/0378-1119(92)90698-O