Gene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment

• Published in: Viruses Vol. 12; no. 11; p. 1284
• Main Authors: Ferreira, Jorge G G, Gava, Sandra G, Oliveira, Eneida S, Batista, Izabella C A, Fernandes, Gabriel da R, Mourão, Marina M, Calzavara-Silva, Carlos E
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 10-11-2020; MDPI AG
• Subjects: acetaminophen; Acetaminophen - pharmacology; Analgesics, Non-Narcotic - pharmacology; Animals; Cell Line; Dengue; Dengue Virus - drug effects; DENV; hepatocytes; Hepatocytes - drug effects; Hepatocytes - virology; Homeostasis - drug effects; Host Microbial Interactions - drug effects; Host Microbial Interactions - genetics; Liver - cytology; Liver - drug effects; Liver - virology; Mice; RNA sequencing (RNAseq); Sequence Analysis, RNA; Transcriptome; Virus Replication - drug effects; RNA sequencing (RNAseq); acetaminophen; DENV; Dengue; transcriptome; hepatocytes
• ISSN: 1999-4915; 1999-4915
• DOI: 10.3390/v12111284

Dengue is an acute viral disease caused by
(DENV) and is considered to be the most common arbovirus worldwide. The clinical characteristics of dengue may vary from asymptomatic to severe complications and severe organ impairment, particularly affecting the liver. Dengue treatment is palliative with acetaminophen (APAP), usually known as Paracetamol, being the most used drug aiming to relieve the mild symptoms of dengue. APAP is a safe and effective drug but, like dengue, can trigger the development of liver disorders. Given this scenario, it is necessary to investigate the effects of combining these two factors on hepatocyte homeostasis. Therefore, this study aimed to evaluate the molecular changes in hepatocytes resulting from the association between DENV infection and treatment with sub-toxic APAP concentrations. Using an in vitro experimental model of DENV-2 infected hepatocytes (AML-12 cells) treated with APAP, we evaluated the influence of the virus and drug association on the transcriptome of these hepatocytes by RNA sequencing (RNAseq). The virus-drug association was able to induce changes in the gene expression profile of AML-12 cells and here we highlight and explore these changes and its putative influence on biological processes for cellular homeostasis.