Identification, sequence analysis, and characterization of cDNA clones encoding two granzyme-like serine proteinases from rat duodenum

Clones of cDNA encoding two serine proteinases were isolated from a cDNA library prepared from rat duodenum mRNA. The deduced amino acid sequences consisted of 248 residues and possessed a high level of homology to one another and to the sequences of granzymes, cathepsin G, and mast cell proteases I...

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Bibliographic Details
Published in:	FEBS letters Vol. 324; no. 2; pp. 226 - 230
Main Authors:	Amerik, Alexander Yu, Yarovoi, Serge V., Grigorenko, Vitali G., Antonov, Vladimir K.
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 14-06-1993 Elsevier
Subjects:	Amino Acid Sequence Animals Base Sequence CAT G, cathepsin G cDNA cloning CHYM A, chymotrypsinogen A Cloning, Molecular DUO-DEN, duodenase Duodenum Duodenum - enzymology Gene Expression GLP I and II, granzyme-like proteins I and II GRAN B, granzyme B Granzyme Molecular Sequence Data PCR, polymerase chain reaction Rats RMCP I and II, rat mast cell proteases I and II RNA, Messenger - genetics Sequence Analysis, DNA Sequence Homology, Amino Acid Serine Endopeptidases - biosynthesis Serine Endopeptidases - genetics Serine proteinase Tissue Distribution CAT G, cathepsin G RMCP I and II, rat mast cell proteases I and II CHYM A, chymotrypsinogen A DUO-DEN, duodenase cDNA cloning Duodenum Granzyme PCR, polymerase chain reaction Serine proteinase GLP I and II, granzyme-like proteins I and II GRAN B, granzyme B
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Summary:	Clones of cDNA encoding two serine proteinases were isolated from a cDNA library prepared from rat duodenum mRNA. The deduced amino acid sequences consisted of 248 residues and possessed a high level of homology to one another and to the sequences of granzymes, cathepsin G, and mast cell proteases I and II. Analysis of the enzymes' primary structures allowed the identification of the catalytic amino acid triad and the prediction of the substrate specificity. Northern blotting experiments showed that while one of these proteinases is expressed only in duodenum, the other enzyme is present in duodenum, lung, and spleen. It is supposed that these proteinases may play an important role in the function of an organism's defence systems.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(93)81398-J