Production of transglutaminase from Bacillus circulans on solid-state and submerged cultivations

• Published in: Biotechnology letters Vol. 25; no. 23; pp. 2029 - 2033
• Main Authors: DE BARROS SOARES, Luis Henrique, ASSMANN, Francine, ZACHIA AYUB, Marco Antonio
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01-12-2003; Springer Nature B.V
• Subjects: Bacillus - classification; Bacillus - growth & development; Bacillus - metabolism; Bacillus circulans; Biological and medical sciences; Bioreactors - microbiology; Biotechnology; Cell Culture Techniques - methods; Cultivars; Cultivation; Enzymatic activity; Enzyme Activation; Fundamental and applied biological sciences. Psychology; Glycine max - chemistry; Immersion; Industrial Waste - prevention & control; Refuse Disposal - methods; Solid state physics; Soybeans; Species Specificity; Transglutaminases - biosynthesis; Transglutaminases - chemistry; transglutaminase; Bacillus circulant; Bacillus circulans; Enzyme; Transferases; Bacillaceae; Aminoacyltransferases; Solid state; solid-state cultivation; submerged cultivation; Bacillales; Submerged culture; Production; Bacteria; Protein-glutamine γ-glutamyltransferase
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1023/B:BILE.0000004397.43058.3e

A Bacillus circulans strain, isolated in the Amazon basin, produced a transglutaminase (EC 2.3 x 2.13) in both submerged and solid-state cultivation. Enzyme activity in the former case reached 0.69 U ml(-1) after 160 h cultivation on starch based medium, and in the latter 0.61 U ml(-1) after 60 h cultivation on soybean industrial fibrous residue, a non-expensive agro-industrial residue.