Design and optimization of an enzymatic membrane reactor for tetracycline degradation

• Published in: Catalysis today Vol. 236; pp. 146 - 152
• Main Authors: de Cazes, M., Belleville, M.-P., Petit, E., Llorca, M., Rodríguez-Mozaz, S., de Gunzburg, J., Barceló, D., Sanchez-Marcano, J.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 01-11-2014; Elsevier
• Subjects: Active membrane; Catalysis; Chemical Sciences; Chemistry; Colloidal state and disperse state; Enzymatic membrane reactor; Exact sciences and technology; General and physical chemistry; Laccase; Membranes; Micropollutant degradation; Tetracycline; Theory of reactions, general kinetics. Catalysis. Nomenclature, chemical documentation, computer chemistry; Wastewater treatment; Micropollutant degradation; Enzymatic membrane reactor; Active membrane; Wastewater treatment; Laccase; Tetracycline; Degradation; Heterogeneous catalysis; Membrane reactor; Membrane; Waste water purification; Optimization
• ISSN: 0920-5861; 1873-4308
• DOI: 10.1016/j.cattod.2014.02.051

•Laccase grafting onto ceramic supports for active membrane elaboration.•Impact of different parameters on laccase activity.•Tetracycline degradation by free and immobilized laccases in osmosed water.•Stability of active membranes for tetracycline degradation in an enzymatic membrane reactor.
The tetracycline, antibiotic considered as a recalcitrant pollutant, was successfully depleted from model aqueous solutions by immobilized laccase from Trametes versicolor in an enzymatic membrane reactor. The results obtained show that tetracycline is depleted from water solutions at room temperature and without adding any extra chemicals. The degradation of tetracycline in aqueous solutions at 20mgL−1 during 24h, with equivalent amounts of free or immobilized biocatalyst, allowed reaching a tetracycline degradation yield of 56% with an enzymatic membrane whereas it was only of 30% with free laccase. This result highlights the good reactivity and stability of the immobilized enzyme for the degradation of tetracycline. Moreover, the enzymatic membrane reactor was able to reach a constant degradation rate of 0.34mg of tetracycline per hour during 10 days.