Expression and clinical significance of basic transcription factor 3 in nasopharyngeal carcinoma

• Published in: Oncology letters Vol. 17; no. 1; pp. 789 - 796
• Main Authors: Chen, Ping, Zhong, Qi, Li, Zufei, Zhang, Yang, Huang, Zhigang
• Format: Journal Article
• Language: English
• Published: Greece Spandidos Publications 01-01-2019; Spandidos Publications UK Ltd; D.A. Spandidos
• Subjects: Apoptosis; Cancer; Care and treatment; Cell cycle; Cell growth; Clinical significance; Committees; Development and progression; Gene expression; Genetic aspects; Head & neck cancer; Health aspects; Laboratories; Lymphatic system; Medical prognosis; Metastasis; Nasopharyngeal cancer; Oncology; Polymerase chain reaction; Proteins; RNA polymerase; Squamous cell carcinoma; Studies; Throat cancer; Transcription factors; Beijing China; United States > US; China; Japan; cell function; small interfering RNA; head and neck squamous cell carcinomas; basic transcription factor 3
• ISSN: 1792-1074; 1792-1082
• DOI: 10.3892/ol.2018.9699

Basic transcription factor 3 (BTF3), a transcription factor and modulator of apoptosis, is differentially expressed in carcinoma. To acquire further understanding of the involvement of BTF3 in carcinoma, the present study analyzed the expression of BTF3, as well as its role in cell function in nasopharyngeal carcinoma (NPC). BTF3 transcription rates in human NPC samples (n=46) and adjacent normal tissue samples (n=46) were analyzed using reverse transcription-quantitative polymerase chain reaction and immunohistochemistry. BTF3-silencing in NPC cells was performed via specific small interfering RNA molecules. The function of BTF3 was analyzed by proliferation assays and colony forming assays using a Cellomic assay system. The positive expression rates of BTF3 were significantly increased in cancerous tissues compared with those in adjacent tissues (P<0.05). In addition, BTF3-silencing decreased cell proliferation and colony formation (P<0.01) in TCA-8113 and 5-8F cells. BTF3 is overexpressed in NPC, and its silencing is associated with decreased cell proliferation and colony formation, enhanced apoptosis and cell cycle regulation of TCA-8113 and 5-8F cells.